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Primary Antibodies  >  Cell Cycle Protein Antibodies
 

p53 Antibody

Mouse Monoclonal BP53-12 IgG2a kappa
Code

200-301-174
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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E-mail: orders@rockland-inc.com
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Immunofluorescence microscopy of HeLa cells using anti-p53. Rockland's Protein A purified Mab anti-p53 was used at a 1:100 dilution in 10% normal goat serum in PBS and reacted overnight at 4° C. After washes cells were incubated with a 1:500 dilution of AlexaFluor?594 Goat-a-Mouse IgG diluted in normal goat serum for 1 h at room temperature. Personnel Communication. Kuldeep Patel, Loyola University.
Western blotting using anti-p53. HeLa whole cell lysate (lane 1), cytosol fraction (lane 2) and nuclear extract (lane 3) (15 ?g) were separated by 10% SDS-PAGE and transferred to nitrocellulose membrane. The membrane was blocked with 3% milk/TBST for 1 h at room temperature followed by incubation with Rockland's Protein A purified Mab anti-p53 overnight at 4° C diluted 1:1,500 in blocking solution. The membrane was washed 3X with TBST and then incubated with a 1:2,000 dilution of HRP Goat-a-Mouse IgG diluted in blocking buffer for 1 h at room temperature. After final washes the proteins reactive on the membrane were detected using ECL. Other detection systems will yield similar results. Personnel Communication. Kuldeep Patel, Loyola University.
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Synonyms: p53 Tumor Suppressor antibody, Phosphoprotein p53 antibody, TP53 antibody, Transformation related protein 53 antibody, TRP53 antibody, Tumor protein p53 antibod

p53 Antibody Properties

Description
Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174
Host
Mouse
Target Species
Human
Known Cross Reactivity
human
Clonality
Monoclonal BP53-12 IgG2a kappa
Application
ELISA : 1:2,000 - 1:10,000
ChIP : 1 µg/µL at 4° o/n
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:50
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.5 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
P53
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Lot#  

p53 Antibody Description

Background
The p53 gene like the Rb gene, is a tumor suppressor gene, i.e., its activity stops the formation of tumors. If a person inherits only one functional copy of the p53 gene from their parents, they are pre-disposed to cancer and usually develop several independent tumors in a variety of tissues in early adulthood. This condition is rare, and is known as Li-Fraumeni syndrome. However, mutations in p53 are found in most tumor types, and so contribute to the complex network of molecular events leading to tumor formation. In most transformed and tumor cells the concentration of p53 is increased 5-1000 fold over the minute concentrations (1000 molecules cell) in normal cells, principally due to the increased half-life (4 h) compared to that
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human p53 protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
This antibody is suitable for ChIP, flow cytometry, immunohistochemistry, immunoblotting and immunoprecipitation.  p53 is the most commonly mutated gene in spontaneously occurring human cancers. Mutations arise with an average frequency of 70% but incidence varies from zero in carcinoid lung tumors to 97% in primary melanomas.  High concentrations of p53 protein are transiently expressed in human epidermis and superficial dental fibroblasts following mild ultraviolet irradiation. This antibody reacts with an N-terminal epitope of the 53 kD gene product and this epitope is not destroyed by formalin-fixation and routine paraffin embedding. Microwaving is needed for optimal staining.
Purity/Specifity
This protein A purified mouse monoclonal antibody reacts specifically with p53 in human tissues and cell lines.  The antibody recognizes a 53 kDa band corresponding to p53.  Cross reactivity with p53 from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Gurney, E. G. et al (1980) Monoclonal antibodies against simian virus 40 T antigens: evidence for distinct sublcasses of large T antigen and for similarities among nonviral T antigens. J Virol, 34(3):752-63. Hollstein, M, et al. (1991) p53 mutations in human cancers. Science, 253: 49-53. Lane, D.P. (1992) p53, guardian of the genome. Nature, 358(6381):15-16
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IP
Reacts With
Human
Catalog Number
100-401-156

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat
Catalog Number
200-301-400

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human, mouse, rat, bovine
Catalog Number
200-401-222

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry