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Primary Antibodies > Infectious Disease Antibodies

H5N1 Antibody VN04-8

Mouse Monoclonal 3G2 IgG1 kappa

Number:

200-301-976

Size:

100 µg

Price:

$349.00

Shipping:

$41.00

Handling:

$20.00

Availability:

Ships next business day.

Datasheet
References
Reviews
Protocols
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H5N1 Antibody VN04-8 Properties

Description

H5N1 Antibody (VN04-8) - 200-301-976

Host

Mouse

Target Species

H5N1 virus

Known Cross Reactivity

virus

Clonality

Monoclonal 3G2 IgG1 kappa

Application

ELISA : 1:5,000
Neutralization : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized

Physical State

Liquid (sterile filtered)

Shipping Condition

Dry Ice

Format

IgG1

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

Relevant Links

NCBI - 58618437
NCBI - 159144921
UniProtKB - A8UDQ2

Download Datasheet

Download CofA:

Lot#

H5N1 Antibody VN04-8 Description

Background

Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) Antibody raised against the hemagglutinin (HA) surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus. Generally referred to as ''bird flu'', the H5N1 influenza A virus has been documented in poultry and humans across ten Eurasian countries, from Japan in the north to Indonesia in the south. Without immunity, humans would have no protection against H5N1 influenza viruses, which could potentially cause a catastrophic pandemic influenza. This antibody, directed against the HA surface glycoprotein of the A/Vietnam/1203/04 (H5N1) influenza virus, is intended to further our understanding of the mechanisms underlying antigenic variation and evolution of novel variants. The major functions of HA include receptor-binding and fusion activities, but there may also be a structural role for HA in viral particle formation. Following attachment of HA to surface receptors on susceptible cells, the influenza virus enters the cell via endocytosis and membrane fusion.

Synonyms

mouse anti-H5N1 Antibody, mouse anti-VN04-8 Antibody, H5HA antibody, Hemagglutinin 5 antibody, H5N1 antibody

Immunogen

Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) monoclonal antibody was produced by intraperitoneal immunization of BALB/c mice with concentrated purified virus preparation containing hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)] using the modification of the method described by Kohler and Milstein. Each mouse received two immunizations of 15 µg HA with incomplete Freund’s adjuvant, administered 3 week apart.

Immunogen Type

Native Protein

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Hemagglutinin of A/Vietnam/1203/04 Influenza Virus (VN04-8) monoclonal antibody can be used for hemagglutination inhibition (HI) assays to provide antigenic characterization of the influenza A viruses of the H5 HA subtype. This monoclonal antibody is suitable for virus neutralization assays (in cell culture and in embryonated chicken eggs), ELISA, immunoprecipitation, immunohistochemistry and western blotting.

Purity/Specifity

This product was purified from tissue culture supernatant fluid by Protein A chromatography and is specific for H5 hemagglutinin (HA) protein of influenza A virus [strain A/Vietnam/1203/04 (H5N1)]. VN04-8 monoclonal antibody did not cross-react with influenza viruses of other HA subtypes. This monoclonal antibody reacted with H5N1 influenza viruses representatives of different clades and subclades of the H5 HA subtype.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference

Guan, Y., et al. (2004) H5N1 Influenza: A Protean Pandemic Threat. Proc. Natl. Acad. Sci. U.S.A. 101: 8156–8161. Li, K. S., et al. (2004) Genesis of a Highly Pathogenic and Potentially Pandemic H5N1 Influenza Virus in Eastern Asia. Nature 430: 209–213. Stevens, J., et al. (2006) Structure and Receptor Specificity of the Hemagglutinin from an H5N1 Influenza Virus. Science 312: 404–410. Hatta, M., et al. (2001) Molecular Basis for High Virulence of Hong Kong H5N1 Influenza A Viruses. Science 293: 1840–1842. Webster, R.G., et al. (1980) The Mechanism of Antigenic Drift in Influenza Viruses: Analysis of Hong Kong (H3N2) Variants with Monoclonal Antibodies to the Hemagglutinin Molecule. Ann NY Acad Sci. 354:142-161.

Specific Reference

Govorkova, E.A., et al. (2006) Immunization with Reverse-Genetics–Produced H5N1 Influenza Vaccine Protects Ferrets against Homologous and Heterologous Challenge. J. Infect. Dis. 194:159–167. Hoffmann, E., et al. (2005) Role of Specific Hemagglutinin Amino Acids in the Immunogenicity and Protection of H5N1 Influenza Virus Vaccines. Proc. Natl. Acad. Sci. 102(36): 12915–12920. ML Reed, HL Yen, RM DuBois, OA Bridges, R Salomon, RG Webster, CJ Russell. (2009). Amino Acid Residues in the Fusion Peptide Pocket Regulate the pH of Activation of the H5N1 Influenza Virus Hemagglutinin Protein. J Virol. 2009 April; 83(8): 3568:3580. BJ Hanson, A Boon, APC Lim, A Webb, EE Ooi, RJ Webby. (2006). Passive immunoprophylaxis and therapy with humanized monoclonal antibody specific for influenza A H5 hemagglutinin in mice. Respiratory Research 2006, 7:126.