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Recombinant Green Fluorescent Protein (GFP) Control Protein - 000-001-215

Anti-GFP (MOUSE) Monoclonal Antibody DyLight™ 488 Conjugated - 200-341-215

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GFP and Epitopes > Antibodies to GFP and Antibodies to RFP

GFP Antibody Dylight™ 800 Conjugated

Mouse Monoclonal 9F9.F9 IgG1 kappa

Number:

200-345-215

Size:

100 µg

Price:

$349.00

Shipping:

$32.00

Handling:

$20.00

Availability:

Ships next business day.

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GFP Antibody Dylight™ 800 Conjugated Properties

Description

Anti-GFP (MOUSE) Monoclonal Antibody DyLight™ 800 Conjugated - 200-345-215

Host

Mouse

Known Cross Reactivity

wt, rGFP, eGFP

Clonality

Monoclonal 9F9.F9 IgG1 kappa

Application

FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : 1:10,000 - 1:25,000
Other Dilution: User Optimized

Epitope Tag Type

GFP

Physical State

Lyophilized

Shipping Condition

Ambient

Format

IgG1

Label

DyLight™ 800

FP Value

2.8 moles DyLight™ 800 per mole of IgG1

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

100 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

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Lot#

GFP Antibody Dylight™ 800 Conjugated Description

Background

GFP Dylight™ 800 Conjugated Antibody is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Synonyms

mouse anti-GFP Antibody Dylight™ 800 Conjugation, Dylight™ 800 Conjugated mouse anti-GFP Antibody, GFP, Green Fluorescent Protein, GFP antibody, Green Fluorescent Protein antibody, EGFP, enhanced Green Fluorescent Protein, Aequorea victoria, Jellyfish

Immunogen

BALB/c mice were immunized with a Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246 aa) derived from the jellyfish Aequorea victoria.

Immunogen Type

Recombinant Protein

Storage Condition

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specifity

GFP Dylight™ 800 Conjugated Antibody was prepared from tissue culture supernatant by Protein A affinity chromatography. Assay by Immunoelectrophoresis resulted in a single precipitin arc against anti-Mouse Serum. Reactivity is observed against recombinant Green Fluorescent Protein (000-001-215, recombinant GFP from Aequorea victoria) by Western blot. No reaction is seen against RFP.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference