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Datasheet

B-Phycoerythrin Antibody

Rabbit Polyclonal

3 References
200-4199 200-401-099S 200-401-099
50 mg 25 µL 100 µg
ELISA, IHC, IF, Multiplex, Other
Phycoerythrin
Rabbit
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Background

Anti-B-Phycoerythrin Antibody detects the B-PE phycoerythrin protein. Phycoerythrin is a red protein from the light-harvesting phycobiliprotein family, present in cyanobacteria, and red algae. Phycoerythrin is organized in a hexameric structure of alpha and beta chains, covalently binding chromophores called phycobilins. R-Phycoerythrin and B-Phycoerythrin are among the brightest biological fluorescent dyes and are commonly used as a fluorescent dye in many assays and is frequently conjugated to antibodies as a reporter molecule. The spectral properties of B-Phycoerythrin are absorption at 545 nm and emission at 572 nm.

Product Details

Anti-B-PHYCOERYTHRIN (RABBIT) Antibody (BULK ORDER) - 200-4199
rabbit anti-B-Phycoerythrin Antibody, rabbit anti B-Phycoerythrin, rabbit anti Phycoerythrin
Rabbit
Polyclonal
IgG

Target Details

cpeA, cpeB
Phycoerythrin
Native Protein
Phycoerythrin [Porphyridium creuentum]
Anti-B-Phycoerythrin Antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Rabbit Serum as well as purified and partially purified Phycoerythrin [Porphyridium creuentum].

Application Details

ELISA
IF, IHC, Other, Multiplex  - View References
Anti-B-Phycoerythrin antibody has been tested by ELISA and is suitable for western blot, immunohistochemistry, immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
5.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
(). Marginal zone SIGN-R1+ macrophages are essential for the maturation of germinal center B cells in the spleen. Proc Natl Acd Sci USA.
Applications
Immunization
(). The opposing forces of shear flow and sphingosine-1-phosphate control marginal zone B cell shuttling. Nat Commun.
Applications
IHC, ICC, Histology
(). Long-term B cell depletion in murine lupus eliminates autoantibody-secreting cells and is associated with alterations in the kidney plasma cell niche. J Immunol.
Applications
IF, Confocal Microscopy; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.