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Datasheet

Cyclin L1 Antibody

Rabbit Polyclonal

200-401-881
500 µg
WB, IHC
Human
Rabbit
$525.00 /Per Item
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Background

Cyclin L (also referred to as CCNL) is encoded by two highly related genes Cyclin L1 and Cyclin L2 (CCNL1 and CCNL2, respectively). Cyclin L has been shown to associate with the PITSLRE kinase and is involved in pre-mRNA processing. Both Cyclin L gene products can be alternatively spliced to produce two isoforms (known as 1 and 2 or alpha and beta). Cyclin L2 is ubiquitous, expressed at much higher levels than Cyclin L1, and thus is likely the major partner for the CDK11p110 protein kinase.

Product Details

Anti-Cyclin L1 (isoform 1) (RABBIT) Antibody - 200-401-881
rabbit anti-Cyclin L1 antibody, Cyclin-L, Cyclin L beta, Cyclin L gamma, CCNL1
Rabbit
Polyclonal
IgG

Target Details

CCNL1  - View All CCNL1 Products
Human
Conjugated Peptide
This Protein A purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic polypeptide corresponding to an internal region near amino acids 300-375 of Human Cyclin L1a protein.
The product was purified from monospecific antiserum by Protein A chromatography. This antibody is specific for human Cyclin L1a and shows negligible reactivity to Cyclin L1b or Cyclin L2 isoforms.  A BLAST analysis was used to suggest reactivity with this protein from human and chimpanzee based on 100% homology for the immunogen sequence. Cross reactivity with Cyclin L1a protein from dog, rat, mouse and chicken is expected, as this sequence shows 87-96% homology with the protein from these sources. Cross reactivity with Cyclin L1a homologues from other sources has not been determined.

Application Details

IHC, WB
This Protein A purified antibody has been tested for use in ELISA, immunohistochemistry, western blot, and immunoprecipitation.   Specific conditions for reactivity should be optimized by the end user.  Expect a band ~ 59 kDa in size corresponding to Cyclin L1a by western blotting in the appropriate cell lysate or extract.

Formulation

Lyophilized
5.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None
100 µL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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