TEV Protease Antibody
Rabbit Polyclonal
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Background
TEV protease, encoded by the Tobacco Etch Virus (TEV), is a 27 kDa catalytic domain of the Nuclear Inclusion a (NIa) protein encoded by the virus (TEV). It is widely used for cleaving fusion proteins because of its sequence specificity. It recognizes a linear epitope of the general form E-Xaa-Xaa-Y-Xaa-Q-(G/S). Cleavage occurs between Q and G or Q and S. The structure of TEV protease is similar to that of the serine protease family. Like serine proteases, TEV protease utilizes a catalytic triad of residues to hydrolyze peptide bonds. The distinguishing feature of TEV protease, however, is that instead of the serine nucleophile in the triad Ser-Asp-His, there is a cysteine, which may explain the resistance of TEV protease to protease inhibitors which are commonly used. The strain used is the auto inactivation-resistant mutant S219V. The catalytic activity of the S219V mutant is approximately 2 fold greater than that of the wild-type protease.
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