Datasheet

TNF alpha Antibody

Name: TNF alpha Antibody
Brand: Rockland Immunochemicals
Price: 475.0 USD
Availability: InStock

Rabbit Polyclonal

2 References

Item No. 210-401-321

Size: 1 mg

Applications: WB, IHC

Reactivity: Mouse

Host Species: Rabbit

$1,225.00 /Per Item

25 µL $125.00

100 µL $475.00

1 mg $1,225.00

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Background

TNF alpha Antibody detects TNF-a protein. TNF-a is a cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is a potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Anti-TNF-alpha Antibody is ideal for investigators involved in Cell Signaling, Immunology and Signal Transduction research.

Expand Background

Specifications for TNF alpha Antibody

Product Details

Description: Anti-Mouse TNF-α (RABBIT) Antibody - 210-401-321

Synonyms: rabbit anti-TNF alpha antibody, rabbit anti-TNFa antibody, rabbit anti-tumor necrosis factor alpha antibody, APC1 antibody, Cachectin antibody, DIF antibody, Differentiation inducing factor antibody, Macrophage cytotoxic factor antibody, MCF antibody, Necrosin antibody, TNF-alpha, Tumor necrosis factor ligand superfamily member 2, TNF-a, TNFA

Host Species: Rabbit

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: Tnf - View All Tnf Products

Reactivity: Mouse

Immunogen Type: Recombinant Protein

Immunogen: This antibody was produced from whole rabbit serum prepared by repeated immunizations with recombinant mouse TNFα produced in E.coli.

Purity/Specificity: TNF alpha Antibody has been heated to 56°C for 30 minutes. The antibody is directed against mature 17,000 MW mouse TNFα and is useful in determining its presence in various assays. The antibody does not recognize mouse TNFß (lymphotoxin).

Database Links:

NCBI - P06804.2

UniProtKB - P06804

GeneID - 21926

Application Details

Tested Applications: WB

Suggested Applications: IHC - View References

Application Note: This antibody against Mouse TNFα has been tested for use in immunoblotting. This antibody is suitable for ELISA, immunohistochemistry, neutralizations, radioimmunoassay, and immunoprecipitation. It recognizes the 17,000 MW TNFα. Reactivity in other immunoassays is unknown. This antibody will recognize the cell-bound precursor of TNFα as a 26,000 protein in immunoblots, particularly in denatured samples. This antibody is also useful for neutralization of mouse activity in bioassays. It does not neutralize the biological activity of lymphotoxin. For neutralization, it is recommended to incubate the sample with a 1:200 dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended.

Formulation

Physical State: Liquid (sterile filtered)

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Sodium Azide

Stabilizer: None

Shipping & Handling

Shipping Condition: Dry Ice

Storage Condition: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (2)

Bretland KA et al. (2021). Irisin treatment lowers levels of phosphorylated tau in the hippocampus of pre‐symptomatic female but not male htau mice. Neuropathol Appl Neurobiol.

Applications

WB, IB, PCA

PubMed

Wang et al. (2018). Metastatic cancers promote cachexia through ZIP14 upregulation in skeletal muscle. Nature Medicine

Applications

IHC, ICC, Histology

PubMed

Related Protocols

Adherent Cell Lysis Protocol

Fluorescent Western Blotting Protocol

Heat-induced Antigen Retrieval Protocol

Histone Immunoblotting Protocol

Immunohistochemistry (IHC) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Protease-induced Antigen Retrieval Protocol

Staining Paraffin Sections by PAP Procedure

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.