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MIP 3 alpha Antibody

Rabbit Polyclonal
NCI Collaboration
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Western blot using Rockland's anti-Mouse MIP3a antibody shows detection of a band ~11 kDa in size corresponding to recombinant mouse MIP3a.  Recombinant mouse MIP-3a was loaded on to an SDS-PAGE gel at 0.25 µg and after separation was transferred to nitrocellulose.  The membrane was blocked with 1% BSA in TBST for 30 min at RT, followed by incubation with primary antibody diluted 1:1,000 in 1% BSA in TBST overnight at 4°C.  After washes, the blot was reacted with secondary antibody HRP Goat anti-Rabbit IgG antibody diluted 1:40,000 in blocking buffer (p/n MB-070) for 30 min at RT followed by reation with FemtoMax™ Chemiluminescent substrate (p/n FEMTOMAX-110).  Data was collected using Bio-Rad VersaDoc® 4000 MP imaging system.
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Number:
210-401-B51
Size:
100 µg
Price:
$319.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

MIP 3 alpha Antibody Properties

Description
Anti-Mouse MIP-3a (RABBIT) Antibody - 210-401-B51
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:10,000
Western Blot : 1:1,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
None
Stabilizer
None
GeneName
Ccl20
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MIP 3 alpha Antibody Description

Background
MIP-3a (also known as C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1) is a chemotactic factor that attracts lymphocytes and, slightly, neutrophils, but not monocytes. MIP-3a inhibits proliferation of myeloid progenitors in colony formation assays and may be involved in formation and function of the mucosal lymphoid tissues by attracting lymphocytes and dendritic cells towards epithelial cells. C-terminal processed forms have been shown to be equally chemotactically active for leukocytes. MIP-3a also possesses antibacterial activity against E.coli and S.aureus. MIP-3a is a secreted protein that is expressed predominantly in the liver, lymph nodes, appendix, peripheral blood lymphocytes, and fetal lung. Low levels of expression are also seen in thymus, prostate, testis, small intestine and colon. C-terminal processed forms which lack 1, 3 or 6 amino acids are produced by proteolytic cleavage after secretion from peripheral blood monocytes.
Synonyms
MIP-3a , CCL20, C-C motif chemokine 20, small-inducible cytokine A20, macrophage inflammatory protein 3 alpha, MIP-3-alpha, liver and activation-regulated chemokine, CC chemokine LARC and beta chemokine exodus-1
Immunogen
This purified antibody was prepared from whole rabbit serum produced by repeated immunizations with full length recombinant mouse MIP-3a protein.
Immunogen Type
Recombinant Protein
Storage Condition
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
This purified antibody has been tested for use in ELISA and western blotting. Reactivity is also expected in neutralizations, radioimmunoassay and immunohistochemistry. The endotoxin content is estimated to be <10 pg/µl by the LAL method. By western blot a band approximately 10.7 kDa in size corresponding to native mouse MIP-3a protein is expected in the appropriate cell lysate or extract. Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
This Protein A purified antibody was heated to 56°C for 30 minutes. In ELISA and other immunoreactive assays, this antibody will recognize both native and recombinant mouse MIP-3a in cell supernatants and certain body fluids. A control of similarly diluted normal rabbit IgG is recommended.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Harper,E.G., Guo,C., Rizzo,H., Lillis,J.V., Kurtz,S.E., Skorcheva,I., Purdy,D., Fitch,E., Iordanov,M. and Blauvelt,A. (2009) Th17 cytokines stimulate CCL20 expression in keratinocytes in vitro and in vivo: implications for psoriasis pathogenesis. J. Invest. Dermatol. 129 (9), 2175-2183. Reboldi,A., Coisne,C., Baumjohann,D., Benvenuto,F., Bottinelli,D., Lira,S., Uccelli,A., Lanzavecchia,A., Engelhardt,B. and Sallusto,F. (2009) C-C chemokine receptor 6-regulated entry of TH-17 cells into the CNS through the choroid plexus is required for the initiation of EAE. Nat. Immunol. 10 (5), 514-523. Sirard,J.C., Didierlaurent,A., Cayet,D., Sierro,F. and Rumbo,M. (2009) Toll-like receptor 5- and lymphotoxin beta receptor-dependent epithelial Ccl20 expression involves the same NF-kappaB binding site but distinct NF-kappaB pathways and dynamics. Biochim. Biophys. Acta 1789 (5), 386-394.
Specific Reference
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Product Type
Proteins and Peptides;
Reacts With
Mouse
Catalog Number
010-001-B51

Product Type
Primary Antibodies;
Applications
WB
Reacts With
human
Catalog Number
209-301-B51

Product Type
Primary Antibodies;
Applications
ELISA, WB, Neu
Reacts With
human
Catalog Number
209-401-B51

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, rat
Catalog Number
210-403-B51

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry