Shopping Cart (0 Items)

My Rockland

Log In | Register | Contact Us

800.656.7625

Advanced Search

You'll need this too:

Anti-p53 (MOUSE) Monoclonal Antibody - 200-301-174

Anti-MAPKAP Kinase 2 (RABBIT) Antibody - 600-401-281

Anti-Histone H2AvD pS137 (RABBIT) Antibody - 600-401-914

Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated - 611-1302

Primary Antibodies > Chromatin Antibodies

HDAC-1 (C-terminus) Antibody

Rabbit Polyclonal

Number:

600-401-879

Size:

100 µg

Price:

$349.00

Shipping:

$41.00

Handling:

$20.00

Availability:

Ships next business day.

Datasheet
References
Reviews
Protocols
Related Products

HDAC-1 (C-terminus) Antibody Properties

Description

Anti-HDAC-1 (RABBIT) Antibody - 600-401-879

Host

Rabbit

Target Species

Human

Known Cross Reactivity

human

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:50,000
IF Microscopy : 10ug / ml
Western Blot : 1:1,000 - 1:5,000
Immunohistochemistry: 1:200 - 1:1,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping Condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.33 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

HDAC1

Relevant Links

UniProtKB - Q13547
NCBI - 13128860
GeneID - 3065

Download Datasheet

Download CofA:

Lot#

HDAC-1 (C-terminus) Antibody Description

Background

HDAC-1 antibody recognizes HDAC1 (also known as HD1, histone deacetylase 1, RPD3, RPD3L1) which belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Synonyms

rabbit anti-HDAC-1 antibody, HDAC1, HDAC 1, HD 1 antibody, histone deacetylase 1 antibody, Histone deacetylase-1, HD1 antibody, RPD3L1, reduced potassium dependency yeast homolog like 1 antibody

Immunogen

Anti-HDAC-1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 466-482 of Human HDAC-1.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-HDAC-1 Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Specific nuclear staining is observed by IHC. Expect bands at 65 kDa in size corresponding to HDAC-1 by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

Anti-HDAC-1 antibody is directed against human HDAC-1 protein. HDAC-1 antibody was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, mouse, rat and chimpanzee sources based on 100% homology for the immunogen sequence. Cross reactivity may occur with HDAC-1 from bovine (82% homology) and chicken (80% homology) sources. Cross reactivity with HDAC-1 homologues from other sources has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference

Unoki,M., Nishidate,T. and Nakamura,Y. (2004) ICBP90, an E2F-1 target, recruits HDAC1 and binds to methyl-CpG through its SRA domain. Oncogene 23 (46), 7601-7610. Zhao,Q., Cumming,H., Cerruti,L., Cunningham,J.M. and Jane,S.M. (2004) Site-specific acetylation of the fetal globin activator NF-E4 prevents its ubiquitination and regulates its interaction with the histone deacetylase, HDAC1. J. Biol. Chem. 279 (40), 41477-41486. Ammanamanchi,S. and Brattain,M.G. (2004) Restoration of transforming growth factor-beta signaling through receptor RI induction by histone deacetylase activity inhibition in breast cancer cells. J. Biol. Chem. 279 (31), 32620-32625.

Specific Reference

Nohata N, Hanazawa T, Kinoshita T, Inamine A, Kikkawa N, Itesako T, Yoshino H, Enokida H, Nakagawa M, Okamoto Y, Seki N. (2013) Tumour-suppressive microRNA-874 contributes to cell proliferation through targeting of histone deacetylase 1 in head and neck squamous cell carcinoma. Br J Cancer. 2013 Apr 30;108(8):1648-58. doi: 10.1038/bjc.2013.122. Epub 2013 Apr 4.