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Primary Antibodies  >  Apoptosis Antibodies
 

Caspase-12 Antibody

Rabbit Polyclonal
Code

600-401-986
Size

100 µg
Price

$309.00
Availability

Ships next business day.
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Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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Western blot analysis of Caspase-12 in human (A) and mouse (B) spleen tissue lysates with Caspase-12 antibody at 1 µg/ml.
Immunohistochemistry of Caspase-12 in human heart tissue with Caspase-12 antibody at 10 µg/ml.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: UNQ9415 antibody, Apoptosis related cysteine protease antibody, CASP 12 antibody, casp12 antibody, CASP12P1 antibody, Caspase 12 pseudogene 1 antibody, Caspase12 antibody, Casp-12

Caspase-12 Antibody Properties

Description
Anti-CASPASE-12 (RABBIT) Antibody - 600-401-986
Host
Rabbit
Target Species
Mouse
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : User Optimized
Western Blot : 0.5-1 µg/ml
Immunohistochemistry: 10 µg/ml
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
0.5 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
CASP12
Download CofA
Lot#  

Caspase-12 Antibody Description

Background
Three distinct signaling pathways lead to programmed cell death (apoptosis). The death receptor and mitochondrion pathways are the mains, in which the key apoptotic proteases capase-8 and caspase-9, respectively, are involved. The endoplasmic reticulum (ER) stress is the third apoptotic pathway and caspase-12 is involved. Caspase-12 is localized to the ER but not to cytoplasm or mitochondrion. Caspase-12 is activated by ER stress, including disruption of ER calcium homeostasis, and mediates ER stress-induced apoptosis. Caspase-12 is co-localized to the ER with several proteins that are involved in Alzheimer's disease including gamma-secretase presenilin and beta-amyloid precursor protein (APP). Caspase-12 mediates cytotoxicity induced by amyloid-beta. Caspase-12 is ubiquitously expressed in mouse tissues. Anti-Caspase-12 antibody is ideal for investigators involved in Apoptosis research.
Immunogen
Caspase-12 Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids near the amino terminus of mouse Caspase-12 protein.
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-Caspase-12 Antibody has been tested for use in ELISA, western blotting, and immunohistochemistry.  Murine spleen tissue lysate can be used as a positive control in western blotting, and a band at approximately 48 kDa is expected.  Specific conditions for reactivity should be optimized by the end user.
Purity/Specifity
Anti-Caspase-12 Antibody is directed against mouse Caspase-12 protein.  The product was affinity purified from monospecific antiserum by immunoaffinity chromatography.  The antibody is human, mouse and rat reactive.  Reactivity against homologues from other sources has not been determined.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
1. Nakagawa T, Zhu H, Morishima N, Li E, Xu J, Yankner BA, Yuan J (2000) Caspase-12 mediates endoplasmic-reticulum-specific apoptosis and cytotoxicity by amyloid-beta. Nature 403:98-103. 2. Mehmet H. Caspases  (2000) find a new place to hide. Nature 403:29-30. 3. Van de Craen M, Vandenabeele P, Declercq W, Van den Brande I, Van Loo G, Molemans F, Schotte P, Van Criekinge W, Beyaert R, Fiers W (1997) Characterization of seven murine caspase family members. FEBS Lett. 403: 61-69.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
209-401-306

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
mouse
Catalog Number
210-401-321

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
mouse, rat, guinea pig
Catalog Number
600-401-972

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry