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Datasheet

ATDC Antibody

Rabbit Polyclonal

600-401-B15S 600-401-B15
25 µL 100 µg
WB, ELISA
Human
Rabbit
$125.00 /Per Item
$475.00 /Per Item
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Background

Ataxia-telangiectasia group D-associated protein (ATDC), also called tripartite motif-containing protein 29 (TRIM29), is a novel Histone deacetylase (HDAC) associated protein. Its function is tightly regulated by HDAC. ATDC Lysine 116 (K116) is acetylated and has a significant functional role in regulating cell survival and tumorigenesis. ATDC is expressed in placenta, prostate and thymus, and is over expressed in pancreatic and cervical tumors. Its function in tumor cells is not fully understood. It is constitutively phosphorylated by PKC on serine/threonine in A431 cells. The ATDC gene product is one of a group of proteins that share multiple zinc finger motifs and an adjacent leucine zipper motif. These proteins have been proposed to form homo- or heterodimers involved in nucleic acid binding, consistent with the fact that many of these proteins appear to be transcriptional regulatory factors involved in carcinogenesis and/or differentiation. The likelihood that the ATDC gene product is involved in transcriptional regulation could explain the pleiomorphic characteristics of AT, including abnormal cell cycle regulation.

Product Details

Anti-ATDC (RABBIT) Antibody - 600-401-B15
rabbit anti-ATDC Antibody, Ataxia-telangiectasia group D associated protein antibody, FLJ36085 antibody, TRIM 29 antibody, Tripartite motif containing protein 29 antibody, TRIM-29
Rabbit
Polyclonal
IgG

Target Details

TRIM29  - View All TRIM29 Products
Human
Conjugated Peptide
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a peptide corresponding to an internal portion of human ATDC protein around lysine 116.
This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with over-expressed, acetylated and non-acetylated (at K116) ATDC protein.  A BLAST analysis was used to suggest cross-reactivity with ATDC from human, horse, bovine, chimpanzee and macaque based on a 100% homology with the immunizing sequence.  Partial reactivity is expected against rat and mouse ATDC based on 92% homology with the immunizing sequence.  Cross-reactivity with ATDC from other sources has not been determined.

Application Details

ELISA, WB
This affinity purified antibody has been tested for use in ELISA and western blotting.   Specific conditions for reactivity and detection of ATDC should be optimized by the end user.  Expect a band approximately ~66 kDa in size corresponding to ATDC by western blotting in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
0.67 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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