BrdU Antibody
Immunofluorescence Microscopy
Bromodeoxyuridine molecule
Immunofluorescence Microscopy - Rabbit anti-BrdU
Immunohistochemistry of Anti-BrdU
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Datasheet

BrdU Antibody

Rabbit Polyclonal

24 References
600-401-C29S 600-401-C29
25 µL 100 µg
WB, ELISA, IHC, IF, FC, Multiplex
BrdU
Rabbit
$125.00 /Per Item
$475.00 /Per Item
25 µL $125.00
100 µg $475.00
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Background

Bromodeoxyuridine (5-bromo-2'-deoxyuridine, BrdU) is a synthetic thymidine nucleoside analog. BrdU is commonly used to allow the detection of growing or proliferating cells in living tissues. During the S-phase of cell division, DNA replication occurs, and BrdU can be incorporated into the newly synthesized DNA by substituting for naturally occurring thymidine. Antibodies specific for BrdU are subsequently used to detect the incorporated BrdU thymidine analog. This highlights cells that were actively replicating their DNA and is suggestive of actively growing cells. Antibody binding usually requires the DNA to be denatured, typically by exposing the cells to acid or heat.

Product Details

Anti-BrdU (RABBIT) Antibody - 600-401-C29
rabbit anti-BrdU Antibody, bromodeoxyuridine, 5-bromo-2'-deoxyuridine
Rabbit
Polyclonal
IgG

Target Details

BrdU
Other
Anti-BrdU affinity purified antibody was purified from monospecific rabbit antiserum prepared via repeated immunizations with BromodeoxyUridine-KLH.
Anti-BrdU Antibody was affinity purified from monospecific antiserum by immunoaffinity chromatography.

Application Details

ELISA, IHC, WB
FC, IF, Multiplex  - View References
Anti-BrdU Antibody has been tested by ELISA, WB, IHC, and is suitable for immunofluorescence microscopy and flow cytometry. Specific conditions for reactivity should be optimized by the end user. Expect to detect incorporated BrdU thymidine analog from replicated cells.

Formulation

Liquid (sterile filtered)
1.1 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store BrdU Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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