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GFP and Epitopes  >  Antibodies to FLAG™ and Antibodies to 6XHIS Tags
 

DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated

Rabbit Polyclonal
NCI Collaboration
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DyLight™ dyes can be used for two-color Western Blot detection with low background and high signal.  Anti-tubulin was detected using a DyLight™ 680 conjugate.  Anti-TNFa was detected using a DyLight™ 800 conjugate. The image was captured using the Odyssey® Infrared Imaging System developed by LI-COR.
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Number:
600-445-383
Size:
100 µg
Price:
$319.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated Properties

Description
Antibody for the detection of FLAG™ conjugated proteins (RABBIT) Antibody DyLight™ 800 Conjugated - 600-445-383
Host
Rabbit
Known Cross Reactivity
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Clonality
Polyclonal
Application
ELISA : 1:10,000 - 1:25,000
FLISA : >1:20,000
IF Microscopy : >1:5,000
Western Blot : 1:10,000 - 1:25,000
Other Dilution: User Optimized
Epitope Tag Type
FLAG
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
FP Value
2.0 moles DyLight™ 800 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
100 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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DYKDDDDK Antibody same epitope as Sigma's Anti-FLAG Dylight™ 800 Conjugated Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
This affinity purified antibody was purified from whole rabbit serum prepared by repeated immunizations with the Enterokinase Cleavage Site (ECS) peptide (D-Y-K-D-D-D-D-K) conjugated to KLH using maleimide.  This antibody reacts with FLAGä conjugated proteins.
Immunogen Type
Peptide
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Purity/Specifity
This affinity purified antibody is directed against the FLAGä epitope tag and is useful in determining its presence in over expressed proteins in various assays.  The antibody recognizes the FLAGä epitope tag (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
611-1302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
B304

Product Label

 

Description
DyLight™ 800
Color
Infra-Red
Conjugation Reference
Modified from Thermo Fisher Scientific Inc, Rockford, IL
Molecular Weight
1050
Excitation Wavelength
770
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester