Chicken IgG (H&L) Antibody Peroxidase Conjugated
ELISA results of Goat Anti-Chicken IgG HRP Antibody
Western Blot of Goat Anti-Chicken IgG HRP Antibody
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Datasheet

Chicken IgG (H&L) Antibody Peroxidase Conjugated

Goat Polyclonal

1 References
603-103-002
2 mg
WB, ELISA, Functional Assay
Chicken
Goat
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Background

Anti-Chicken IgG Peroxidase Antibody generated in goat detects chicken IgY. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both heavy and light chains of the antibody molecule are present.

Product Details

Goat Anti-Chicken IgG (H&L) Antibody Peroxidase Conjugated - 603-103-002
Anti-Chicken Antibody, Gt-a-Chicken IgG, Anti-Chicken IgY, gt-a-ch IgY, gt-a-ch IgG, Gt-a-Chicken IgY, Chicken Antibody in Goat, Chicken Secondary Antibody
Goat
IgG (H&L)
Peroxidase (HRP)
Polyclonal
IgG

Target Details

Chicken
Native Protein
Anti-Chicken IgG (H&L) was produced by repeated immunization with Chicken IgG whole molecule in goat.
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Chicken IgG coupled to agarose beads.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum, Chicken IgG and Chicken Serum.

Application Details

ELISA, WB
Functional Assay  - View References
Anti-Chicken IgG (H&L) HRP Conjugated Antibody is tested in immunoelectrophoresis, western-blot, competitive western-blot, ELISA and competitive ELISA assays. Anti-Chicken IgG HRP Antibody is suitable for IHC. Specific conditions for reactivity and signal detection should be optimized by the end user.

Formulation

Lyophilized
2.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.
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Applications
E, EIA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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