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Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated
Goat Polyclonal
46 References
610-145-002
610-145-002-0.5
100 µg
5 x 100 µg
WB, IHC, IF, Dot Blot
Mouse
Goat
Availability: Ships next business day
Availability: Ships next business day
Shipping info:
$50.00 to US & $70.00 to Canada for most products. Final costs are calculated at checkout.
Background
Anti-Mouse IgG DyLight 800 Antibody generated in goat detects reactivity to Mouse IgG. Secreted as part of the adaptive immune response by plasma B cells, immunoglobulin G constitutes 75% of serum immunoglobulins. Immunoglobulin G binds to viruses, bacteria, as well as fungi and facilitates their destruction or neutralization via agglutination (and thereby immobilizing them), activation of the compliment cascade, and opsonization for phagocytosis. The whole IgG molecule possesses both the F(c) region, recognized by high-affinity Fc receptor proteins, as well as the F(ab) region possessing the epitope-recognition site. Both the Heavy and Light chains of the antibody molecule are present. Secondary Antibodies are available in a variety of formats and conjugate types. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.
Product Details
Goat Anti-Mouse IgG (H&L) Antibody DyLight™ 800 Conjugated - 610-145-002
Goat Anti-Mouse IgG Secondary Antibody DyLight™800 Conjugated, Goat Anti-Mouse IgG Antibody DyLight™800 Conjugated, Anti-mouse IgG secondary antibody, anti-mouse IgG DyLight™800 conjugated secondary antibody
Goat
IgG (H&L)
DyLight™ 800
Polyclonal
IgG
1.6
Target Details
Mouse
Mouse IgG whole molecule
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose followed by conjugation to fluorochrome and extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse IgG and Mouse Serum. This antibody will react with heavy chains of Mouse IgG and with light chains of most Mouse immunoglobulins.
Application Details
Dot Blot, WB
IF, IHC
- View References
Anti-Mouse IgG DyLight 800 Antibody has been tested by dot blot and western blot and is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. The emission spectra for this DyLight™ conjugate match the principle output wavelengths of most common fluorescence instrumentation.
Formulation
Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
100 µL
Restore with deionized water (or equivalent)
Shipping & Handling
Ambient
Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
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WB, IB, PCA
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WB, IB, PCA
Orengo, JP et al. (2022). Reduction of mutant ATXN1 rescues premature death in a conditional SCA1 mouse model. JCI Insight
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WB, IB, PCA
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WB, IB, PCA
Nitschke L et al. (2021). Modulation of ATXN1 S776 phosphorylation reveals the importance of allele-specific targeting in SCA1. JCS Insight.
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
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Undefined
Woods BL. et al. (2020). Interplay of septin amphipathic helices in sensing membrane-curvature and filament bundling. bioRxiv
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WB, IB, PCA
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WB, IB, PCA
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IF, Confocal Microscopy
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WB, IB, PCA
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WB, IB, PCA
Nowinski SM et al. (2020). Mitochondrial fatty acid synthesis coordinates oxidative metabolism in mammalian mitochondria. Elife
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
Bacman SR et al. (2020). Manipulation of mitochondrial genes and mtDNA heteroplasmy. Methods Cell Biol.
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WB, IB, PCA
Ma X et al. (2020). Nicotinamide mononucleotide adenylyltransferase uses its NAD+ substrate-binding site to chaperone phosphorylated Tau. Elife.
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WB, IB, PCA
Araiz et al. (2019). Enhanced β-adrenergic signalling underlies an age-dependent beneficial metabolic effect of PI3K p110α inactivation in adipose tissue. Nature Communications
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WB, IB, PCA
Hong D et al. (2019). Deletion of TMEM268 inhibits growth of gastric cancer cells by downregulating the ITGB4 signaling pathway. Cell Death Differ.
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WB, IB, PCA
Yu S et al. (2019). RSRC1 suppresses gastric cancer cell proliferation and migration by regulating PTEN expression. Mol Med Rep.
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WB, IB, PCA
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WB, IB, PCA
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WB, IB, PCA
Subramanian J et al. (2019). CPG15/neuritin mimics experience in selecting excitatory synapses for stabilization by facilitating PSD95 recruitment. Cell Rep.
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WB, IB, PCA
Latorre-Pellicer A et al. (2019). Regulation of mother-to-offspring transmission of mtDNA heteroplasmy. Cell Metab.
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WB, IB, PCA
Campbell LA et al. (2019). Gesicle-mediated delivery of CRISPR/Cas9 ribonucleoprotein complex for inactivating the HIV provirus. Mol Ther.
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WB, IB, PCA
Lin et al. (2018). Liver-specific deletion of Eva1a/Tmem166 aggravates acute liver injury by impairing autophagy. Cell Death & Disease
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IHC, ICC, Histology; WB, IB, PCA
Zhang et al. (2018). Amelioratory Effects of Testosterone Propionate on Age-related Renal Fibrosis via Suppression of TGF-β1/Smad Signaling and Activation of Nrf2-ARE Signaling. Scientific Reports
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WB, IB, PCA
Haapalainen et al. (2018). Expression of CPPED1 in human trophoblasts is associated with timing of term birth. Journal of Cellular and Molecular Medicine
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WB, IB, PCA
Yu S et al. (2018). PPP2R2D, a regulatory subunit of protein phosphatase 2A, promotes gastric cancer growth and metastasis via mechanistic target of rapamycin activation. Int J Oncol.
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WB, IB, PCA
Jarrett KE et al. (2018). Somatic Editing of Ldlr With Adeno-Associated Viral-CRISPR Is an Efficient Tool for Atherosclerosis Research. Arteriosclr Thromb Vasc Biol.
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WB, IB, PCA
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WB, IB, PCA
Bacman SR et al. (2018). MitoTALEN reduces mutant mtDNA load and restores tRNA Ala levels in a mouse model of heteroplasmic mtDNA mutation. Nat Med.
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WB, IB, PCA
Bacman, SR et al. (2018). MitoTALEN reduces mutant mtDNA load and restores tRNAAla levels in a mouse model of heteroplasmic mtDNA mutation. Nature Medicine
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WB, IB, PCA
Jarrett et al. (2017). Somatic genome editing with CRISPR/Cas9 generates and corrects a metabolic disease. Scientific Reports
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WB, IB, PCA
Li et al. (2017). Deletion of Pdcd5 in mice led to the deficiency of placenta development and embryonic lethality. Cell Death & Disease
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WB, IB, PCA
Xia et al. (2017). Knockout of MARCH2 inhibits the growth of HCT116 colon cancer cells by inducing endoplasmic reticulum stress. Cell Death & Disease
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WB, IB, PCA
Shen et al. (2016). EMC6/TMEM93 suppresses glioblastoma proliferation by modulating autophagy. Cell Death & Disease
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WB, IB, PCA
Xia D et al. (2016). MARCH2 regulates autophagy by promoting CFTR ubiquitination and degradation and PIK3CA-AKT-MTOR signaling. Autophagy.
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WB, IB, PCA
Wang Z et al. (2014). PHF23 (plant homeodomain finger protein 23) negatively regulates cell autophagy by promoting ubiquitination and degradation of E3 ligase LRSAM1. Autophagy.
Applications
IP, Co-IP
Li Y et al. (2013). A novel ER-localized transmembrane protein, EMC6, interacts with RAB5A and regulates cell autophagy. Autophagy.
Applications
WB, IB, PCA
Viorela Pop et al. (2013). Early brain injury alters the blood-brain barrier phenotype in parallel with β-amyloid and cognitive changes in adulthood. J Cereb Blood Flow Metab.
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WB, IB, PCA
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
