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Protein A Purification

NCI Collaboration
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Antibody-binding protein specificity for Protein A, Protein G, and Protein A/G which demonstrate binding specificity to IgG immunoglobulin through an interaction in the Fc region of the heavy chain domain. Protein A and G each show some preferences to certain types of antibody subclasses, and proper reagent selection may be required, Protein A/G offers the widest range of antibody subclass binding. Another immunoglobulin binding protein is Protein L which binds to the kappa light chain of the F(ab’)2 region.
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Number:
CUST22C
Size:
1 Each
Price:
Price On Request
Availability:
Ships within 2 weeks.

Protein A Purification Properties

Description
PROTEIN A PURIFICATION (up to 100 ml serum or 100 ml of ascites or 500 ml of processed and concentrated tissue culture supernate) - CUST22C
Shipping Condition
Wet Ice
Label
Concentration
2.19mg/ml by UV absorbance at 280 nm
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Protein A Purification Description

Background
Protein A is 56 kDa protein molecule found in the cell wall of the bacterium Staphylococcus aureus. Protein A binds to immunoglobulins through interaction with the IgG heavy chain within the Fc region. Protein A does not bind to all IgG's equivalently and binding varies among species. Even within a species, Protein A interacts with some subclasses of IgG and not others. Protein A is well suited for purification of most rabbit, mouse, rat, and certain human IgG class of immunoglobulin. Rockland offers Protein G or recombinant Protein A/G when recommended by Rockland for optimal purifcation.
Synonyms
purification, IgG fraction, antibody purification
Application Note
Protein A purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein A purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. The IgG is bound to an immobilized Protein A matrix, non-IgG impurities are wased away, lastly the IgG fraction is eluted and recovered. Rockland's Protein A purification process is ideal to recover a highly active IgG fraction from complex starting material.
Purity/Specifity
Chromatography is performed on up to 100 mL of antiserum, ascites or concentrated tissue culture supernatant to purify immunoglobulins known to bind (including certain mouse subclasses) Protein A.
General Reference
Differences in anti-protein A activity among IgG subgroups. J Immunol 103(4):828-33; Kronvall, G. and Williams, R.C. (1969) Protein A: Nature's universal anti-body. Trends in Biochem Sci 7: 74-76. Surolia, A., et al. (1982).
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry