Protein A purification is a process for isolating the IgG type of immunoglobulin protein from a complex mixture. The starting material is usually but not limited to polyclonal antisera, tissue culture supernatant, or ascites fluid. Typically protein A purification is performed when IgG enrichment is desired, and is often used to purify IgG prior to conjugation to a reporter molecule. The IgG is bound to an immobilized Protein A matrix, non-IgG impurities are wased away, lastly the IgG fraction is eluted and recovered. Rockland's Protein A purification process is ideal to recover a highly active IgG fraction from complex starting material.
Chromatography is performed on up to 100 mL of antiserum, ascites or concentrated tissue culture supernatant to purify immunoglobulins known to bind (including certain mouse subclasses) Protein A.