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Custom Polyclonal Antibody Production

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Custom Polyclonal Antibody Production Properties

ELISA : User Optimized
ChIP : User Optimized
IF Microscopy : User Optimized
Neutralization : User Optimized
Flow Cytometry : User Optimized
Western Blot : User Optimized
Immunohistochemistry: User Optimized
ImmunoPrecipitation: User Optimized
Other Dilution: User Optimized
Physical State
Shipping Condition
Wet Ice
0.01% (w/v) Sodium Azide
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Custom Polyclonal Antibody Production Description

If you have a target protein or other immunogen, Rockland's team of experts can generate polyclonal antisera. Our scientists can consult with you on your antigen, choice of host, and other specific details such as host animal pre-screening or ELISA testing of the generated antisera to determine specificity. Special targets such as post-translational modificaitons such as a phosphorylated epitope or epigenetic modification. An agreement is put into place. Options include providing the immunogen or having Rockland produce the peptide or protein. An injection schedule will be provided but may be customized. Typically a 28-day Test bleed will be shipped to you for analysis. Add-on services are available such as: recombinant protein generation, custom vialing, lyophilization, ELISA, western blot, purification (protein A or G, affinity, cross-adsorption (for example, to non-phosphopeptide), conjugation (to reporter/dye), assay development, Host Cell Protein generation, stability study, test material manufacture, and others. As an option to be discussed after testing the first bleed, a valuable antibody which should be made available to a great many others in your field may be eligible to be made and marketed in collaboration. Ask also about our antibody generation Packages which can save you time and money.
Custom Antibody, Custom Polyclonal, Antibody Production
Custom; peptide or protein
Application Note
Host animals include: rabbits, goats, sheep, chickens, rats, mice, or guinea pigs.
An immunogen designed to produce specific antibodies is injected into host animals. The animals are bled periodically and the bleeds shipped to be tested in your laboratory. We have the capability to expand on each step depending on your needs. Our specialty is in making and testing antibodies, from designing and producing antigens and test materials through making the purified and conjugated products to designing assays using the new antibodies.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
1. Vaitukaitis, J.L. Production of Antisera with Small Doses of Immunogen: Multiple Intradermal Injections. Methods in Enzymology 73; 46-52, 1981. 2. Harlow, E. and Lane, D. Antibodies: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, NY, 1988.
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Conjugation Reference
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