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Cyclin Antibody Sampler Kit

NCI Collaboration JCYIA Product
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  • Rockland Cyclin Antibody Sampler Kit
  • Anti-Cyclin D1 Antibody - Western Blot
  • Anti-Cyclin A Antibody - Immunohistochemistry
  • Anti-Cyclin B1 Antibody - Immunohistochemistry
Number:
K911
Size:
1 Kit
Price:
$839.00
Shipping:
$41.00
Handling:
$20.00
Availability:
Ships next business day.

Cyclin Antibody Sampler Kit Properties

Description
CYCLIN ANTIBODY SAMPLER KIT - K911
Application
ELISA : 1:2,000 - 1:10,000
Western Blot : 1:500 - 1:5,000
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized
Product Type
Kit
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Label
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Cyclin Antibody Sampler Kit Description

Background
Cyclin A is a member of the cyclin family. Cyclin A binds to S phase Cdk2 and is required for the cell to progress through the S phase. Cyclin A/ Cdk2 is inhibited by the complex p21CIP. Cyclin B1 is a regulatory protein involved in mitosis. The gene product forms a complex with p34 (cdc2) to form the maturation-promoting factor (MPF). Two alternative transcripts have been found, a constitutively expressed transcript and a cell cycle-regulated transcript that is expressed predominantly during G2/M phase. The different transcripts result from the use of alternate transcription initiation sites. Cyclin D1 (also known as G1/S-specific cyclin D1, PRAD1 oncogene, BCL-1 oncogene, and PRAD1: parathyroid adenomatosis 1) is encoded by a gene that belongs to the highly conserved cyclin family. Cyclins are characterized by a dramatic periodicity in protein abundance throughout the cell cycle and function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns, which contribute to the temporal coordination of each mitotic event. Cyclin D1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. This protein has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Cyclin E is a member of the cyclin family. Cyclin E binds to the G1 phase Cdk2, which is required for the transition from G1 to S phase of the cell cycle that determines cell division. The Cyclin E/CDK2 complex phosphorylates p27Kip1, tagging it for degradation, thus promoting expression of Cyclin A, allowing progression to S phase. Anti-Cyclin Antibodies are ideal for investigators involved in Cell Signaling, cell biology and Signal Transduction research.
Synonyms
Anti-Cyclin A Antibody, Anti-Cyclin B1 Antibody, Anti-Cyclin D1 Antibody, Anti-Cyclin E Antibody, Goat Anti-Rabbit IgG Antibody, antibody sample kit
Storage Condition
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Application Note
Each kit contains one unit of the following products: (100 µL) Anti-Cyclin A (RABBIT) Antibody (100 µL) Anti-Cyclin B1 (RABBIT) Antibody (100 µL) Anti-Cyclin D1 (RABBIT) Antibody (100 µL) Anti-Cyclin E (RABBIT) Antibody (100 µg) Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, mouse, rat
Catalog Number
100-401-151

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human, rat, mouse
Catalog Number
100-401-152

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-401-153

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human
Catalog Number
100-401-156

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry