6X His Epitope Tag Antibody
Anti-6X His Antibody - Western Blot
WB - 6xHIS Epitope Tag Antibody
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Datasheet

6X His Epitope Tag Antibody

Rabbit Polyclonal

19 References
600-401-382S 600-401-382
25 µL 100 µg
WB, ELISA, IHC, Dot Blot, ChIP, IP, Multiplex
His-Tag
Rabbit
6X His Epitope Tag Antibody Peroxidase Conjugated 6X His Tag Antibody Biotin Conjugated 6X His Tag Antibody Dylight™ 488 Conjugated 6X His Tag Antibody Dylight™ 549 Conjugated 6X His Tag Antibody Dylight™ 649 Conjugated 6X His Tag Antibody Dylight™ 680 Conjugated 6X His Tag Antibody Dylight™ 800 Conjugated 6X His Tag Antibody Dylight™ 405 Conjugated
$125.00 /Per Item
$279.00 /Per Item
25 µL $125.00
100 µg $279.00
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Background

Epitope tags are short peptide sequences that are easily recognized by tag-specific antibodies.  Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties.   Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins containing the epitope tag sequence.  This allows Anti epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.  This means that anti-epitope tag antibodies are a useful alternative to generating specific antibodies to identify, immunoprecipitate or immunoaffinity purify a recombinant protein.  The anti-epitope tag antibody is usually functional in a variety of antibody-dependent experimental procedures.  Expression vectors producing epitope tag fusion proteins are available for a variety of host expression systems including bacteria, yeast, insect and mammalian cells.  Rockland Immunochemicals produces anti-epitope tag antibodies against many common epitope tags including Myc, GST, GFP, 6X His, MBP, FLAG and HA. Rockland Immunochemicals also produces antibodies to other tags including FITC, Rhodamine (TRITC), DNP and biotin.

Product Details

Anti-6X His Epitope Tag (RABBIT) Antibody - 600-401-382
rabbit anti-6X His Epitope Tag Antibody, anti-HIS, HIS Antibody, 6X His Tag Antibody, HHHHHH epitope tag antibody
Rabbit
Polyclonal
IgG

Target Details

His-Tag
Conjugated Peptide
This antibody was purified from whole rabbit serum prepared by repeated immunizations with 6X His epitope tag peptide H-H-H-H-H-H conjugated to KLH using maleimide.
This affinity purified antibody is directed against the 6X His motif and is useful in determining its presence in various assays. This polyclonal anti-6X His tag antibody detects over-expressed proteins containing the 6X His epitope tag.  To date, this antibody has reacted with all His tagged proteins so far tested.  In western blotting of bacterial extracts, the antibody does not cross-react with endogenous proteins.  The antibody recognizes the His-tag (His-His-His-His-His-His) fused to either the amino- or carboxy- termini of targeted proteins in transfected or transformed cells.

Application Details

Dot Blot, ELISA, WB
ChIP, IHC, IP, Multiplex  - View References
Anti-6X His is optimally suited for monitoring expression of His-tagged fusion proteins. As such, anti-6X His/6X His can be used to identify fusion proteins that contain the 6X His epitope.  The antibody recognizes the His tag fused either to the amino- or carboxy- termini of targeted proteins.  This antibody has been tested by ELISA and western blotting against both the immunizing peptide and His-containing recombinant proteins.  Although not tested, this antibody is likely functional for immunoprecipitation and immunocytochemistry.

Formulation

Liquid (sterile filtered)
1.22 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). A Dual Regulatory Role of the PhoU Protein in Salmonella Typhimurium. MBio
Applications
WB, IB, PCA
(). The induction of natural competence adapts staphylococcal metabolism to infection. Nature Communications
Applications
WB, IB, PCA
(). Effectors with chitinase activity (EWCAs), a family of conserved, secreted fungal chitinases that suppress chitin-triggered immunity. Plant Cell.
Applications
WB, IB, PCA
(). Growth-regulated Hsp70 phosphorylation regulates stress responses and prion maintenance. Mol Cell Biol.
Applications
WB, IB, PCA
(). Intellectual disability-associated UNC80 mutations reveal inter-subunit interaction and dendritic function of the NALCN channel complex. Nat Commun.
Applications
WB, IB, PCA
(). Nonimmune cell-derived ICOS ligand functions as a renoprotective αvβ3 integrin-selective antagonist. Journal of Clinical Investigation
Applications
WB, IB, PCA
(). Conjugation of an scFab domain to the oligomeric HIV envelope protein for use in immune targeting. PloS One
Applications
WB, IB, PCA
(). USP7 is a SUMO deubiquitinase essential for DNA replication. Nature Structural & Molecular Biology
Applications
IP, Co-IP
(). Expression of Recombinant Cellulase Cel5A from Trichoderma reesei in Tobacco Plants. J Vis Exp.
Applications
WB, IB, PCA
(). Targeted delivery of ubiquitin-conjugated BH3 peptide-based Mcl-1 inhibitors into cancer cells. Bioconjugate Chemistry
Applications
Cellular assay; WB, IB, PCA
(). Effect of the GPI anchor of human Thy-1 on antibody recognition and function. Lab Invest.
Applications
WB, IB, PCA
(). The prolyl isomerase Pin1 acts synergistically with CDK2 to regulate the basal activity of estrogen receptor α in breast cancer. PLoS One
Applications
WB, IB, PCA
(). Characterization of Novel OTases as Tools for Glycoengineering. Glycobiology
Applications
WB, IB, PCA
(). The chromatin-remodeling enzymes BRG1 and CHD4 antagonistically regulate vascular Wnt signaling. Mol Cell Biol.
Applications
ChIP
(). Androgen receptor serine 81 mediates Pin1 interaction and activity. Cell Cycle
Applications
WB, IB, PCA
(). Distinct functional domains contribute to degradation of the low density lipoprotein receptor (LDLR) by the E3 ubiquitin ligase inducible Degrader of the LDLR (IDOL). J Biol Chem.
Applications
WB, IB, PCA
(). Bone morphogenetic protein 15 and growth differentiation factor 9 expression in the ovary of European sea bass (Dicentrarchus labrax): cellular localization, developmental profiles, and response to unilateral ovariectomy. Gen Comp Endocrinol.
Applications
WB, IB, PCA
(). Nectin-like molecules/SynCAMs are required for post-crossing commissural axon guidance. Development.
Applications
IHC, ICC, Histology; Multiplex Assay
(). Immunological applications of single-domain llama recombinant antibodies isolated from a naive library. Protein Eng Des Sel.
Applications
WB, IB, PCA

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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