House Phospho Banner
 
You may also want to consider these related products.
ATM pS1981 Peptide - 000-000-400
ATM pS1981 Peptide - 000-000-400
Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 100-401-264
Anti-NFKB p65 (Rel A) pS276 (RABBIT) Antibody - 100-401-264
Anti-SMC1 pS957 (MOUSE) Monoclonal Antibody - 200-301-397
Anti-SMC1 pS957 (MOUSE) Monoclonal Antibody - 200-301-397
Anti-ATM Protein Kinase S1981 (RABBIT) Antibody - 600-401-398
Anti-ATM Protein Kinase S1981 (RABBIT) Antibody - 600-401-398
Primary Antibodies  >  Sample Size Primary Antibodies
 

ATM pS1981 Antibody

Mouse Monoclonal 10H11.E12 IgG1 kappa
Code

200-301-400S
Size

25 µL
Price

$95.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
Bulk Orders:
Call or E-mail Us For Information
Fill out our Contact Form
Anti ATM antibody showing overlay of anti-ATM pS1981 staining. Cells were fixed 15 min after 5 Gy (IR+) of irradiation, then labeled with antibody. See Kitagawa et al. for additional details.
Anti ATM Mab with human derived HEK293 cells treated with doxorubicin using Rockland's Protein A Purified Mab anti-ATM Protein Kinase pS1981(clone 10H11.E12).  A 370 kDa band corresponding to phosphorylated ATM is detected (arrowhead, lane 2). The lysate was prepared with HALT phosphatase inhibitor (Pierce).   Pre-incubation of peptide with 50 µg of immunizing phospho peptide negates specific staining (lane 1).  Approximately 30 µg of lysate was added to each lane of an SDS-PAGE gel under non-reducing conditions.  The protein was transferred to nitrocellulose using standard methods.  After blocking the membrane was probed with the primary antibody diluted 1:500 overnight at 4°C followed by washes and reaction with a 1:10,000 dilution of IRDye™800 conjugated Gt-a-Mouse IgG [H&L] (code 610-132-121) for 40 min at room temperature.  LICOR's Odyssey® Infrared Imaging System was used to scan and process the image.  Other detection systems will yield similar results.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: DKFZp781A0353 antibody, Human phosphatidylinositol 3 kinase homolog antibody, MGC74674 antibody, Serine protein kinase ATM antibody, T cell prolymphocytic leukemia antibody

ATM pS1981 Antibody Properties

Description
Anti-ATM Protein Kinase pS1981 (MOUSE) Monoclonal Antibody - 200-301-400S
Host
Mouse
Known Cross Reactivity
human, mouse, rat
Clonality
Monoclonal 10H11.E12 IgG1 kappa
Application
ELISA : 1:20,000 - 1:100,000
IF Microscopy : 1:100 - 1:500
Western Blot : 1:200 - 1:2,000
Immunochemistry: Not Recommended
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ATM
Download CofA
Lot#  

ATM pS1981 Antibody Description

Background
Anti ATM pS1981 Antibody is a phospho site specific antibody and recognizes the product of the ATM gene that is mutated in the hereditary disease ataxia-telangiectasia. ATM codes for a protein kinase that acts as a master regulator of cellular responses to DNA double-strand breaks. ATM is normally inactive and the question of how it is activated in the event of DNA damage (due to ionizing radiation for instance) is central to understanding its function. ATM protein is now shown to be present in undamaged cells as an inactive dimer. Low doses of ionizing radiation, which induce only a few DNA breaks, activate at least half of the total ATM protein present, possibly in response to changes in chromatin structure. The ATM gene encodes a 370-kDa protein that belongs to the phosphoinositide 3-kinase (PI(3)K) superfamily, but which phosphorylates proteins rather than lipids. The 350-amino-acid kinase domain at the carboxy terminus of this large protein is the only segment of ATM with an assigned function. Exposure of cells to IR triggers ATM kinase activity, and this function is required for arrests in G1, S and G2 phases of the cell cycle. Several substrates of the ATM kinase participate in these IR-induced cell-cycle arrests. These include p53, Mdm2 and Chk2 in the G1 checkpoint; Nbs1, Brca1, FancD2 and SMC1 in the transient IR-induced S-phase arrest; and Brca1 and hRad17 in the G2/M checkpoint. Ideal for Cancer, Cell Signaling, Chromatin, Neuroscience and Signal Transduction research.
Immunogen
Anti-ATM phospho S1981 Antibody was produced from a synthetic peptide S-L-A-F-E-E-G-Sp-Q-S-T-T-I-S-S corresponding to aa 1974-1988 of human ATM.
Storage Condition
Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
Application Note
Protein A Purified Mab anti-ATM has been tested by ELISA and western blotting against both the native and recombinant forms of the protein. The antibody immunoprecipitates ATM from irradiated human and mouse cells. By immunofluoresence, foci are detected in irradiated human and mouse fibroblasts. This antibody is not recommended for immunohistochemistry. Instead, for IHC, use the clone 7C10D8 (p/n 200-301-500).
Purity/Specifity
Anti-ATM phospho S1981 Monoclonal Antibody antibody is directed against human ATM and is useful in determining its presence in various assays. This monoclonal anti-ATM antibody recognizes the phosphorylated epitope in native and over-expressed proteins found in various tissues and extracts. By ELISA reactivity against SLAFEEGSpQSTTISS at a 1:1600 dilution shows an absorbance >3.000; whereas reactivity against SLAFEEGSQSTTISS shows and absorbance of 0.145. Reactivity is observed against human and mouse ATM. Cross reactivity with ATM from other mammalian sources has not been tested.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Bakkenist, C. J. & Kastan, M. B. (2003). DNA damage activates ATM through intermolecular autophosphorylation and dimer dissociation. Nature 421, 499-506. Kitagawa R, Bakkenist CJ, McKinnon PJ, Kastan MB. (2004) Phosphorylation of SMC1 is a critical downstream event in the ATM-NBS1-BRCA1 pathway. Genes Dev. 18(12):1423-38. Falck, J. Coates, J. and Jackson, S.P. (2005) Conserved modes of recruitment of ATM, ATR and DNA-PKcs to sites of DNA damage. Nature 434: 605-611. Bartkova J, Horejsi Z, Koed K, Kramer A, Tort F, Zieger K, Guldberg P, Sehested M, Nesland JM, Lukas C, Orntoft T, Lukas J, Bartek J. (2005) DNA damage response as a candidate anti-cancer barrier in early human tumorigenesis. Nature 434; 864-870. Bartkova J, Bakkenist CJ, Rajpert-De Meyts E, Skakkebaek NE, Sehested M, Lukas J, Kastan MB, Bartek J. (2005) ATM Activation in Normal Human Tissues and Testicular Cancer. Cell Cycle 4;(6) [Epub ahead of print].
Log in to submit your product review.

Product Type
Proteins and Peptides;
Catalog Number
000-000-400

Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC
Reacts With
human
Catalog Number
100-401-264

Product Type
Primary Antibodies;
Applications
ELISA, WB, IF
Reacts With
human, mouse
Catalog Number
200-301-397

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Human, Mouse
Catalog Number
600-401-398

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry