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Secondary Antibodies > ATTO Conjugated Antibodies

Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed

Goat Polyclonal

ATTO ® dyes can be used for multicolor immunofluorescent detection with low background and high signal. Examples shown are:
A. Tubulin in PtK2- male Rat Kangaroo Kidney Epithelial Cells was detected using ATTO 532 labeled secondary antibody.
B. Muscle alpha-actin was stained with a mouse primary antibody and ATTO 488 anti-mouse IgG (green) while Cytokeratin was stained with polyclonal rabbit anti-cytokeratin and ATTO 647N anti-rabbit IgG (red).
C. HUVEC (Human umbilical vein endothelial cells were stained with anti- Vimentin-ATTO 532 (green), anti-E-Cadherin-ATTO 655 (red) and DAPI (blue).
D. Rat colon sections were stained with Anti-Aquaporin 3-ATTO 594 antibody. Hoechst 33342 (blue) is used as counterstain.
Images provided courtesy of Dr. Jörg Reichwein, ATTO-TEC GmbH

Number:

610-154-042

Size:

500 µg

Price:

$289.00

Shipping:

$40.00

Handling:

$20.00

Availability:

Ships within 2 weeks.

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Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed Properties

Description

Anti-MOUSE IgG2b (Gamma 2b chain) (GOAT) Antibody ATTO 550 Conjugated (Min Cross Reactivity to Bv, Hu, and Rb Serum Proteins) - 610-154-042

Host

Goat

Specificity

IgG2b

Target Species

Mouse

Known Cross Reactivity

Mouse

Clonality

Polyclonal

Application

FLISA : >1:20,000
IF Microscopy : >1:5,000
Flow Cytometry : 1:500 - 1:2,500
Western Blot : >1:10,000
Immunohistochemistry: >1:5,000
Other Dilution: User Optimized

Physical State

Lyophilized

Shipping Condition

Ambient

Format

IgG

Label

ATTO 550

FP Value

2.5 moles ATTO 550 per mole of IgG

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

500 µL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

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Mouse IgG2b Antibody ATTO 550 Conjugated Pre-adsorbed Description

Background

ATTO Dye Conjugated Secondary Antibodies are designed for STED microscopy, FRET, immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms. When choosing a secondary antibody, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment composition.

Synonyms

IgG2b antibody, mouse IgG2b, anti-subclass secondary antibody, AT550, ATTO 550, ATTO-TEC 550

Immunogen

Mouse IgG2b heavy chain

Immunogen Type

Native Protein

Storage Condition

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-Mouse IgG2b secondary antibody is suitable for ELISA, Immunohistochemistry, western blotting as well as other anti IgG2b antibody based assays. The emission spectra for this ATTO conjugate matches the principle output wavelengths of most common fluorescence instrumentation.

Purity/Specifity

Anti-MOUSE IgG2b (Gamma 2b chain) Antibody was prepared from monospecific antiserum by immunoaffinity chromatography using antigens coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Goat Serum, Mouse Serum and Mouse IgG2b. No reaction was observed against Bovine, Human, and Rabbit Serum Proteins. Specificity was confirmed by ELISA at less than 1% of target signal.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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