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Proteins and Peptides  >  Control Peptides
 

DYKDDDDK (FLAG®) Peptide

Code

000-000-383
Size

1.0 mg
Price

$85.00
Availability

Ships next business day.
Order Offline:
Phone: 800.656.7625
E-mail: orders@rockland-inc.com
Fax: 610.367.7825
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SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel.
Lane 1:  Cell lysate before purification. 
Lane 2:  Flow through (used cell lysate).    Lane 3:  Purified DYKDDDDK (FLAG® tag)  recombinant protein (arrowhead).
Load:  (6 µL per lane). 
Predicted/Observed size:  70kDa for DYKDDDDK tagged recombinant protein.
SDS-PAGE of Anti-DYKDDDDK (FLAG® tag) Affinity Gel.
Lane 1:  crude lysate containing over-expressed DYKDDDK-tagged recombinant protein .  
Lane 2:  unbound flow-through of consisting of endogenous E.coli proteins.  Lane 3:  enriched recombinant protein.  
Load:  5 µg protein, 15µL flow through.
Predicted/Observed size:  70kDa for DYKDDDDK tagged recombinant protein.
Shipping & Handling Fees Apply.

Shipping:
$40.00 to United States
Handling:
$20.00
Synonyms: synthetic FLAG® peptide, ddddk peptide, dykddddk peptide, Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys, control peptide, blocking peptide

DYKDDDDK (FLAG®) Peptide Properties

Description
DYKDDDDK (FLAG®) Peptide - 000-000-383
Application
Western Blot : YES as blocking peptide in PCA
ImmunoPrecipitation: YES - see PROTOCOL
Epitope Tag Type
FLAG
Physical State
Lyophilized
Shipping condition
Ambient
PSC Type
Synthetic Peptide
PSC Format
Peptide
Label
Concentration
1 mg/mL by dry weight
Buffer
None
Reconstitution Volume
1.0 mL
Reconstitution Buffer
0.05 M Tris Chloride, 0.15 M Sodium Chloride, pH 7.4
Preservative
None
Stabilizer
None
Download CofA
Lot#  

DYKDDDDK (FLAG®) Peptide Description

Background
The FLAG® peptide (Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys or DYKDDDDK) is intended for immunoaffinity chromatography to allow elution under non-denaturing conditions by competitive elution with excess of free FLAG® Peptide.
Storage Condition
Store vial at 2 - 8 ° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Application Note
A working concentration of 500 µg/mL for triple FLAG® peptide is typically used for competitive elution of FLAG® containing proteins from anti-DYKDDDDK affinity gels (p/n 200-350-383). For use as a control in antibody-peptide competition assays (PCA) use the peptide at 1.0 µg per 1.0 µl of antibody/antiserum in per assay. See anti-DYKDDDDK FLAG® affinity gel PROTOCOL for specific instructions for use.
Purity/Specifity
Greater than 90% specific peptide by HPLC and MS.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
A. Einhauer and A. Jungbauer, (2001), The FLAG® peptide, a versatile fusion tag for the purification of recombinant proteins. J. Biochemical Biophysical Methods, 49 (1); 455–465.
Specific Reference
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Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, FC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
200-301-383

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, FC
Reacts With
Carboxy and amino terminal linked FLAG™ tagged recombinant proteins
Catalog Number
200-301-B13

Product Type
GFP and Epitopes;
Applications
IP
Reacts With
Internal, carboxy and amino terminal linked FLAG® tagged recombinant proteins
Catalog Number
200-350-383

Product Type
Secondary Antibodies;
Applications
ELISA, WB, IHC
Catalog Number
610-4302

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry