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Datasheet

Adenosine Deaminase Antibody Peroxidase Conjugated

Rabbit Polyclonal

200-403-140 200-403-140S 200-403-140-0100
20 mg 25 µL 100 µg
WB, ELISA
Bovine Calf Spleen
Rabbit
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$455.00 /Per Item
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Background

Adenosine deaminase (adenosine aminhydrolase, or ADA) is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. ADA irreversibly deaminates adenosine, converting it to the related nucleoside inosine by the substitution of the amino group for a hydroxyl group. Inosine can then be deribosylated by another enzyme called purine nucleoside phosphorylase (PNP), converting it to hypoxanthine.

Product Details

Anti-Adenosine Deaminase (Calf Spleen) (RABBIT) Antibody Peroxidase Conjugated (BULK ORDER) - 200-403-140
rabbit anti-Adenosine Deaminase Antibody Peroxidase Conjugation, HRP conjugated rabbit anti-Adenosine Deaminase Antibody, Adenosine deaminase, Adenosine aminohydrolase
Rabbit
Peroxidase (HRP)
Polyclonal
IgG

Target Details

ADA  - View All ADA Products
Bovine Calf Spleen
Native Protein
Adenosine Deaminase [Calf Spleen] ADA
Adenosine deaminase is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Rabbit Serum as well as purified and partially purified Adenosine Deaminase [Calf Spleen].  Cross reactivity against Adenosine Deaminase from other sources is unknown.

Application Details

ELISA, WB
Anti-Adenosine deaminase Peroxidase antibody has been tested by ELISA and western blot and is assayed against 1.0 ug of Adenosine Deaminase [Calf Spleen] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) code # ABTS-100 as a substrate for 30 minutes at room temperature.  A working dilution of 1:20,000 to 1:100,000 of the reconstitution concentration is suggested for this product.

Formulation

Lyophilized
10.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
2.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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