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p35 Control Protein

NCI Collaboration
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SDS-PAGE of p35 Control Protein.  
Lane 1:  Molecular Weight Marker.  
Lane 2:  p35 Control Protein.   
Load:  10 µl.
Predicted/Observed size:  69.5 kDa fusion protein, 27.1 kDa for p35, 42.4 kDa for MBP alone.
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Number:
000-001-C12
Size:
100 µg
Price:
$305.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

p35 Control Protein Properties

Description
p35 Control Protein - 000-001-C12
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: Lateral Flow Assay: User Optimized
Species of Origin
Borrelia burgdorferi
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
PSC Type
Protein
PSC Format
Recombinant Protein
Label
Concentration
1.0mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BB-H32, bba64
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p35 Control Protein Description

Background
The p35 kDa protein of the spirochete Borrelia burgdorferi is being investigated for use as an early diagnostic marker of Lyme Disease. Borrelia may change its antigenic composition in its need for adaptation to stresses imposed by changes in conditions from cycling between its arthropod and mammalian hosts. This group of B. burgdorferi proteins may be induced in the tick midgut during the feeding event. The p35 protein elicits a protective immunity from wild type B. burgdorferi. It has been shown that p35 expression in B. burgdorferi is upregulated in the stationary growth phase, and that a temperature of 34°C but not 24°C influenced the expression. The expression of a majority of the proteins expressed in early Lyme disease is affected pH, being abundantly expressed at pH 7.0 (resembling the tick midgut pH of 6.8 during feeding) but only sparsely at pH 8.0 (a condition closer to that of the unfed tick midgut pH of 7.4). The encoding genes may be coregulated. The 35-kDa antigen has been shown to be a statistically significant marker in IgG immunoblots in a study of patients with early Lyme disease who presented with erythema migrans. Recombinant p35 protein may be useful as a diagnostic reagent, especially in combination with other antigens that have been deemed relevant in serodiagnosis of early Lyme disease. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections , autoimmune, and neurodegenerative diseases.
Synonyms
bba64, Borrelia burgdorferi p35, control protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Application Note
p35 is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 69.5 kDa for p35-MBP, (27.1 kDa for p35 and 42.4 kDa for MBP) in size corresponding to p35 by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
p35 is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified p35 and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Gautam, A., Hathaway, M., McClain, N., Ramesh, G., Ramamoorthy, R. (2008). Analysis of the determinants of bba64 (P35) gene expression in Borrelia burgdorferi using a gfp reporter. Microbiology 154: 275-285. Robert D. Gilmore Jr., Rebekah R. Howison, Gabrielle Dietrich, Toni G. Patton, Dawn R. Clifton, and James A. Carroll, (2010). The bba64 gene of Borrelia burgdorferi, the Lyme disease agent, is critical for mammalian infection via tick bite transmission. PNAS vol. 107 no. 16 7515-7520. Fikrig E, Barthold SW, Sun W, Feng W, Telford SR 3rd, Flavell RA. (1997). Borrelia burgdorferi p35 and p37 proteins, expressed in vivo, elicit protective immunity. Immunity 1997 May;6(5):531-9.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Borrelia burgdorferi
Catalog Number
200-401-C12

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry