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OspA Control Protein

NCI Collaboration
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SDS-PAGE of OspA Control Protein.  
Lane 1:  Molecular Weight Marker.  
Lane 2:  OspA Control Protein.   
Load:  10 µl at 1:2 dilution.
Predicted/Observed size:  70.5 kDa fusion protein, 28.1 kDa for OspA, 42.4 kDa for MBP alone.
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Number:
000-001-C13
Size:
100 µg
Price:
$305.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

OspA Control Protein Properties

Description
OspA Control Protein - 000-001-C13
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: Lateral Flow Assay: User Optimized
Species of Origin
Borrelia burgdorferi
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
PSC Type
Protein
PSC Format
Recombinant Protein
Label
Concentration
1.04 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
ospA, BB_A15
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OspA Control Protein Description

Background
Outer-Surface Protein A (OspA), a lipoprotein from Borrelia burgdorferi encoded on its Plasmid lp54, is a major component of the spirochete's extracellular matrix. OspA probably serves as a lipid-anchor. The spirochetes migrate from the tick midgut during feeding to its salivary glands and are thus transmitted to the mammal host. This transition may be facilitated by changes in expression of some B. burgdorferi genes. Upon transmission of the spirochete from the Ixodes tick to mammalian host, the transcript level of OspA can change. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections , autoimmune, and neurodegenerative diseases.
Synonyms
Outer surface protein A, Borrelia burgdorferi OspA, control protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Application Note
OspA is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 70.5 kDa for OspA-MBP, (28.1 kDa for OspA and 42.4 for MBP) in size corresponding to OspA by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
OspA is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified OspA and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Norris SJ. The dynamic proteome of Lyme disease Borrelia. (2006) Genome Biol. 7(3):209. Epub 2006 Mar 17. Ding W., Huang X., Yang X., Dunn J.J., Luft B.J., Koide S., Lawson C.L. (2000) Structural identification of a key protective B-cell epitope in Lyme disease antigen OspA. J. Mol. Biol. 302:1153-1164(2000) [PubMed: 11183781] [Abstract]. Stevenson B, Tilly K, and Rosa P.A. (1996) A Family of Genes Located on Four Separate 32-Kilobase Circular Plasmids in Borrelia burgdorferi B31. J Bacteriol 178(12) June 1996 p3508-3516.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Borrelia burgdorferi
Catalog Number
200-401-C13

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry