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VlsE Control Protein

NCI Collaboration
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Number:
000-001-C33
Size:
100 µg
Price:
$305.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

VlsE Control Protein Properties

Description
VlsE Control Protein - 000-001-C33
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: Lateral Flow Assay: User Optimized
Species of Origin
Borrelia burgdorferi
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
PSC Type
Protein
PSC Format
Recombinant Protein
Label
Concentration
1.0mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
vlsE
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VlsE Control Protein Description

Background
Variable Lipoprotein Surface-Exposed protein, or VlsE, is a lipoprotein on the surface of the Lyme Disease spirochete Borrelia burgdorferi, detectable during all its life stages. It can exist as many different isoforms. VlsE has variable regions (VRs) and invariable regions (IRs). Some IRs are anchored in the outer membrane of the bacteria and some are antigens exposed on the membrane surface. Replacement of the VR by Borrelia within days of being transferred to a mammalian host presents new surface antigens to the host immune system, and helps Borrelia avoid a strong reaction by host immune systems. The VlsE is apparently not modified as much in the tick or in the rodent vector, when compared to in the mammal host. Several putative envelope proteins of B. burgdorferi appear to be expressed only in the infected mammalian host. The VRs are antigenic, irregularly shaped loops on the bacterial surface which may help to hide both membrane-incorporated and surface portions of adjacent proteins from immune cells. These VR loops are coded by antigenic cassettes. The protein loops can therefore be switched in or out of the protein, or different type loops traded. In B. burgdorferi there seem to be at least fifteen different VlsE cassettes that can insert into any of the variable regions of VlsE, allowing it to appear as millions of different antigens. Similar, but smaller, systems also operate for OSP-A, OSP-B, OSP-C, and other proteins. Some current research involves determination of control of cassette activation. One IR region, C6, of the VlsE protein, consistently stimulates a strong immune response. Its presentation may be a decoy that misdirects the immune system from less protected sites by causing competion for binding antibodies. The bound antibodies are thus not available for binding important therapeutic proteins. This may help Borrelia to enter T-cells, leading to their destruction. Because IR6 is invariable and found in all life stages of B. burgdorferi, it has been used in an ELISA diagnostic test for early IgM of Lyme Disease. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections , autoimmune, and neurodegenerative diseases.
Synonyms
Outer surface protein VlsE, Borrelia burgdorferi VlsE, vlsE protein, control protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Application Note
VlsE is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 78.7 kDa for VlsE-MBP, (36.3 kDa for VlsE and 42.4 kDa for MBP) in size corresponding to VlsE by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
VlsE is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified VlsE and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Ohnishi J, Schneider B, Messer WB, Piesman J, and de Silva AM. (2003) Genetic Variation at the vlsE Locus of Borrelia burgdorferi within Ticks and Mice over the Course of a Single Transmission Cycle. J Bacteriol. 185(15): 4432-4441. Magnarelli LA, Lawrenz M, Norris SJ, and Fikrig E. (2002) Comparative reactivity of human sera to recombinant VlsE and other Borrelia burgdorferi antigens in class-specific enzyme-linked immunosorbent assays for Lyme borreliosis. J. Med. Microbiol. (51) 649-655. Kornacki JA and Oliver DB. (1998) Lyme Disease-Causing Borrelia Species Encode Multiple Lipoproteins Homologous to Peptide-Binding Proteins of ABC-Type Transporters. Infection and Immunity 66(9): 4115-4122.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Borrelia burgdorferi
Catalog Number
200-401-C33

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry