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Flagellin Control Protein

NCI Collaboration
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SDS-PAGE of Flagellin Control Protein.  
Lane 1:  Molecular Weight Marker.  
Lane 2:  Flagellin Control Protein.   
Load:  10 µl at 1:6 dilution.
Predicted/Observed size:  76.3 kDa fusion protein, 33.9 kDa for Flagellin, 42.4 kDa for MBP alone.
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Number:
000-001-C14
Size:
100 µg
Price:
$305.00
Shipping:
$20.00
Handling:
$20.00
Availability:
Ships next business day.

Flagellin Control Protein Properties

Description
Flagellin Control Protein - 000-001-C14
Application
ELISA : User Optimized
Western Blot : User Optimized
Other Dilution: Lateral Flow Assay: User Optimized
Species of Origin
Borrelia burgdorferi
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
PSC Type
Protein
PSC Format
Recombinant Protein
Label
Concentration
1.0mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
BBU94A_0149, fla
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Flagellin Control Protein Description

Background
Flagellin is a protein found in the hollow cylinder forming the filament in bacterial flagellum. Its structure is helical, which is important for its function. Studies comparing aflagellate Borrelia to flagellated indicate that the flagella have a role in the invasion of human tissue. The N- and C-termini of flagellin form the inner core of the flagellar filament, and the central portion of the protein makes up the outer surface. While the terminus of the protein is quite similar between all bacterial flagellins, the central portion is variable. The flagellin genes are highly conserved among the different Borrelia species. Mammals often have acquired immune responses (T-cell and antibody responses) to flagellated bacterium. Some bacteria are able to switch between multiple flagellin genes in order to evade this response. Borrelia burgdorferi, the spirochete that is associated with Lyme Disease, may use this tactic when challenging mammals with infection. Borrelia have double-stranded linear plasmids in addition to supercoiled circular plasmids, in low copy number. This suggests that initiation of DNA replication and partitioning are carefully controlled during the cell division cycle. It is believed that expression of the various proteins associated with the spirochete may be regulated by the changes in tick life cycle, changes in conditions during tick feeding (such as temperature, pH, and nutrients) and/or in coordination with the course of infection of the mammal host, i.e., changes in environment as the spirochete migrates from the tick's midgut to its salivary glands to the mammal host. B. burgdorferi can attach to (and also differentially express antigens in) diverse tissues within the vertebrate host and the tick vector, suggesting that physiological factors other than pH and temperature may play roles in modulating B. burgdorferi gene expression. Lyme disease proteins are ideal for researchers interested in immunology, neurology, and rheumatology, coinfections , autoimmune, and neurodegenerative diseases.
Synonyms
41 kDa antigen, Borrelia burgdorferi p41, fla, Flagellar filament 41 kDa core protein, Bacterial flagellin, control protein
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Dilute only prior to immediate use.
Application Note
Flagellin is suitable as a control in immunological assays. Specific conditions for reactivity should be optimized by the end user. Expect a band at 76.3 kDa Flagellin-MBP, (33.9 kDa for Flagellin and 42.4 kDa for MBP) in size corresponding to Flagellin by Western blotting in the appropriate cell lysate or extract.
Purity/Specifity
Flagellin is a fusion protein with an MBP tag and was expressed in E.coli. Analysis by SDS-PAGE resulted in a pattern consistent with purified Flagellin and was estimated to be greater than 95% pure.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Laila Noppa, Nils Burman, Ariadna Sadziene, Alan G. Barbour and Sven Bergstrom (1995) Expression of the flagellin gene in Borrelia is controlled by an alternative c factor. Microbiology 141, 85-93. Wallich R, Moter SE, Simon MM, Ebnet K, Heiberger A, and M D Kramer MD. (1990) The Borrelia burgdorferi flagellum-associated 41-kilodalton antigen (flagellin): molecular cloning, expression, and amplification of the gene. Infect Immun. 58(6): 1711-1719. Panelius J, Lahdenne P, Saxen H, Heikkilä T, and Seppäla I. (2001) Recombinant Flagellin A Proteins from Borrelia burgdorferi Sensu Stricto, B. afzelii, and B. garinii in Serodiagnosis of Lyme Borreliosis. Journal of Clinical Microbiology, November 2001, p. 4013-4019, Vol. 39, No. 11.
Specific Reference
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Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
Borrelia burgdorferi B31
Catalog Number
200-401-C14

Product Type
Supporting Reagents;
Applications
ELISA, WB, IF
Catalog Number
B501-0500

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF
Catalog Number
BSA-50

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry