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Datasheet

GATA4 Antibody

Mouse Monoclonal 10D8.B10.D4 IgG1 kappa

200-301-P15S 200-301-P15
25 µL 100 µg
WB, ELISA
Human
Mouse
$125.00 /Per Item
$485.00 /Per Item
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Background

GATA4, also know as GATA-binding factor 4, acts in cooperation with NKX2-5 as a transcriptional activator of ANF. It binds to the consensus sequence 5'-AGATAG-3' on DNA. GATA4 is critical for the enlargement of cardiac myocytes. Mutations in GATA4 have been linked with congenital heart malfunctions and malformations. Anti-GATA4 antibody is ideal for researchers interested in developmental biology, stem cell research, cell growth and cancer research.

Product Details

Anti-GATA4 (MOUSE) Monoclonal Antibody - 200-301-P15
mouse anti-GATA4 Antibody, Transcription factor GATA-4, GATA-binding factor 4, GATA-4 Antibody
Mouse
Monoclonal
IgG1

Target Details

GATA4  - View All GATA4 Products
Human
Recombinant Protein
GATA4 protein A purified antibody was prepared in mice by repeated immunizations with a recombinant protein corresponding to the first 100 amino acids of human GATA4 protein.
Anti-GATA4 was purified from concentrated tissue culture supernatant Protein A chromatography.   A BLAST analysis was used to suggest reactivity with mouse and rat GATA4 based on a 93% homology. Cross-reactivity with GATA4 from other sources has not been determined.

Application Details

ELISA, WB
Anti-GATA4 antibody has been tested by ELISA and western blotting.  Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 44 kDa in size corresponding to GATA4 by western blotting in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
1.0mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.