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Datasheet

Ataxin 1 Antibody Phycoerythrin

Mouse Monoclonal S65-37 IgG1

200-308-W87
100 µg
WB, IHC
Human, Mouse, Rat
Mouse
$495.00 /Per Item
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Background

Ataxin-1 is a member of the ATXN1 protein family and contains a single AXH domain. It is a neurodegenerative disorder protein thought to have a role in the metabolism of RNA as it has been shown to localize to the RNA and transcription dependent inclusions within the nucleus. A mutation of Ataxin-1 is the cause of spinocerebellar ataxia type-1 (SCA1), a progressive, neurodegenerative disease that is autosomal dominant and primarily affects the Purjinke cells found in brain stem neuronal populations and the cerebellum. Expression of Ataxin-1 is almost ubiquitous, except in the brain where it is isolated to populations of neurons. Anti-Ataxin-1 is ideal for research in Neuroscience.

Product Details

Anti-Ataxin 1 (MOUSE) Monoclonal Antibody Phycoerythrin Conjugated - 200-308-W87
Ataxin-1, ATX1, Atxn1, D6S504E, OTTHUMP00000016065, SCA1, Spinocerebellar ataxia type 1 protein, Spinocerebellar ataxia type 1 protein homolog
Mouse
R-Phycoerythrin (RPE)
Monoclonal
IgG1

Target Details

Ataxin-1  - View All Ataxin-1 Products
Human, Mouse, Rat
Conjugated Peptide
Anti-Ataxin 1 Antibody was produced by repeated immunization of mice with a synthetic peptide containing amino acids 746-761 of mouse ataxin-1.
Anti-Ataxin 1 Antibody was purified from concentrated tissue culture supernate by Protein G chromatography. BLAST analysis suggests that it is 93% identical to rat and 87% identical to human.

Application Details

IHC, WB
Anti-Ataxin 1 R-PE Conjugated Antibody is suitable for Western Blots and Immunocytochemistry. Expect a band approximately ~85kDa on specific lysates or tissues. No cross reactivity against phosphor S751-Ataxin-1. Minimal cross reactivity against S751A mutant of Ataxin-1 by ELISA and immunofluorescence and negative by immunoblot. Specific conditions for reactivity should be optimized by the end user.

Formulation

Liquid (sterile filtered)
1mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.1% (w/v) Sodium Azide
50% (v/v) Glycerol

Shipping & Handling

Wet Ice
Store vial at 4° C prior to opening. Dilute only prior to immediate use. This product is stable at 4° C as an undiluted liquid. Use subdued lighting during handling and incubation of cells prior to analysis. Store reagent in the dark. DO NOT FREEZE.
Expiration date is one (1) year from date of receipt.

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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