JAM A phospho Y280 Antibody
Immunofluorescence Microscopy of Rabbit anti-JAMA pY280 antibody
Western blot for Anti-JAM A pY280 Antibody
Confocal Immunofluorescence Microscopy of Rabbit Anti-JAM-A pY280 antibody of confluent (intestinal epithelial cells) IECs
Confocal Immunofluorescence Microscopy of Rabbit Anti-JAM-A pY280 antibody in polarized epithelial cells
Immunofluorescence Microscopy of Rabbit Anti-JAM-A pY280 antibody with PP2 and pervanadate
Western Blot of Rabbit Anti-JAM-A pY280 antibody with IL-22
Western Blot of Rabbit Anti-JAM-A pY280 antibody with PP2
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Datasheet

JAM A phospho Y280 Antibody

Rabbit Polyclonal

2 References
600-401-GN5S 600-401-GN5
25 µL 100 µg
WB, ELISA, IF, Multiplex
Human
Rabbit
$125.00 /Per Item
$475.00 /Per Item
25 µL $125.00
100 µg $475.00
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Background

Anti-JAM A pY280 was designed, produced, and validated as part of the Joy Cappel Young Investigator Award (JCYIA). JAM A pY280 Antibody detects junctional adhesion molecule A (JAM A) protein phosphorylated at the tyrosine 280 location. JAM A is involved in cell-cell contact and tight junction formation in epithelial cells. JAM A protein is a transmembrane protein expressed in epithelial and endothelial cells as well as in hematopoietic cells and is important for a variety of cellular processes including tight junction assembly, leukocyte transmigration, platelet activation, angiogenesis and virus binding. In addition, JAM A may be a potential therapeutic target for breast cancer.

Product Details

Anti-JAM A pY280 (RABBIT) Antibody - 600-401-GN5
rabbit anti-JAM A pY280 antibody, JAM-A, Junctional adhesion molecule A, JAM-1, Junctional adhesion molecule 1, Platelet F11 receptor, Platelet adhesion molecule 1, PAM-1, CD321, JAM1, JCAM, JAM 1, JAMA
Rabbit
Polyclonal
IgG

Target Details

F11R  - View All F11R Products
Human
Phosphorylation
Conjugated Peptide
Affinity purified Anti-JAM A pY280 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to the c-term and phosphorylated at the tyrosine 280 position of Human JAM A protein.
Anti-JAM A pY280 is directed against human JAM A phosphorylated at the tyrosine 280 position.    This product is an affinity purified antibody produced by immunoaffinity chromatography using phospho peptide coupled to agarose beads followed by solid phase adsorption(s) against non-phospho peptide to remove any unwanted reactivities.  A BLAST analysis was used to suggest reactivity with this protein from human and feline based on 100% homology for the immunogen sequence.
UniProtKB - Q9Y624
NCBI - NP_058642.1
GENEID - 50848

Application Details

ELISA, IF, WB
Multiplex  - View References
This affinity purified antibody has been tested for use in ELISA, IF, and western blot.   Specific conditions for reactivity should be optimized by the end user. Expect a band ~ 32.5 kDa in size corresponding to JAM A by western blotting in the appropriate cell lysate or extract.

Formulation

Liquid (sterile filtered)
1.02mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
None

Shipping & Handling

Dry Ice
Store vial at -20° C prior to opening.  Aliquot contents and freeze at -20° C or below for extended storage.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). Enteropathogenic Escherichia coli (EPEC) Recruitment of PAR Polarity Protein Atypical PKCζ to Pedestals and Cell-Cell Contacts Precedes Disruption of Tight Junctions in Intestinal Epithelial Cells. Int J Mol Sci.
Applications
IF, Confocal Microscopy
(). Role of JAM-A tyrosine phosphorylation in epithelial barrier dysfunction during intestinal inflammation. Molecular Biology of the Cell
Applications
IF, Confocal Microscopy; WB, IB, PCA; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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