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PINK1 truncated Antibody

Rabbit Polyclonal
JCYIA Product
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Number:
600-401-GU5
Size:
100 µg
Price:
$349.00
Shipping:
$41.00
Handling:
$20.00
Availability:
Ships within 2 weeks.

PINK1 truncated Antibody Properties

Description
Anti-PINK1 truncated (RABBIT) Antibody - 600-401-GU5
Host
Rabbit
Target Species
Human
Known Cross Reactivity
Human and Mouse
Clonality
Polyclonal
Application
ELISA : User Optimized
IF Microscopy : 20 µg/mL
Flow Cytometry : User Optimized
Western Blot : 1µg/mL
Immunohistochemistry: 1:200
ImmunoPrecipitation: User Optimized
Physical State
Liquid (sterile filtered)
Shipping Condition
Dry Ice
Format
IgG
Label
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
PINK1
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PINK1 truncated Antibody Description

Background
PINK1 (PTEN induced putative kinase 1) belongs to the protein kinase superfamily and Ser/Thr protein kinase family. PINK1 protects against mitochondrial dysfunction during cellular stress by phosphorylating mitochondrial proteins. It is involved in the clearance of damaged mitochondria via selective autophagy (mitophagy) by mediating activation and translocation of PARK2. Targets PARK2 to dysfunctional depolarized mitochondria through the phosphorylation of MFN2. Activates PARK2 in 2 steps: (1) by mediating phosphorylation at Ser-65 of PARK2 and (2) mediating phosphorylation of ubiquitin, converting PARK2 to its fully-active form. PINK1 interacts with PARK2 and FBXO7 and forms a complexes with PARK2 and PARK7. PINK1 is associated with Parkinson disease 6 (PARK6). Anti-PINK1 Antibody is useful for researcher interested in neurological research, neurodegenerative disorders, Pink/Parkin Mediated Mitophagy, and Parkinsons Disease Pathway.
Synonyms
rabbit anti-PINK1 antibody, Serine/threonine-protein kinase PINK1 (mitochondrial), BRPK, PTEN-induced putative kinase protein 1, PTEN-induced kinase
Immunogen
Anti-PINK1 truncated antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a C-Term portion of human PINK1 conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-PINK1 truncated Antibody has been tested in Western Blot, Immunofluorescence, and ELISA. Positive controls were recombinant PINK1 protein and overexpressed PINK1 transfected HEK293 @ 1µg/mL in western blot. Expect a lower strength band at 62.7 kDa in western blot using appropriate lysates. PINK1 truncated antibody strongly detects endogenously truncated PINK1 protein, seen at ~30kDa. PINK1 is highly phosphorylated and degrades rapidly which may cause additional banding in western blot. Positive control cell lines for immunofluorescence assays are NIH3T3 (p/n W10-000-358). Although not tested, this antibody is likely functional in immunohistochemistry, flow cytometry, and immunoprecipitation.
Purity/Specifity
This affinity purified antibody is directed against human PINK1. This product was affinity purified from monospecific antiserum by immunoaffinity purification. Blast analysis of the sequence of the immunogen shows 100% identity with human and 93% identity to mouse.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
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Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-000-364

Product Type
Cell Lysates;
Applications
WB
Catalog Number
W09-001-372

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry