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The Standard method is a process is where the two sets of animals are immunized using high and low molecular weight Host Cell Protein (HCP) extract. After immunization and primary boosting, antisera is collected. The developed anti-HCP antibodies are evaluated to determine coverage of potential HMW and LMW host cell contaminants . The final HCP antibody product is a pool of anti-HMW and anti-LMW antibodies having broad coverage of the HCP antigens in the final antibody reagent. Coverage is demonstrated by 1D western blot analysis or 2D western Blot Analysis against the HCP extract. HCP-detection assay development is available. To obtain the optimal level of coverage multiple host animals may be utilized. The following species are available; Rabbit, Chicken, Sheep, Goat and Donkey.
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625