Anti-Idiotypic Antibody Production Service



For more than twenty-five years, Rockland Immunochemicals, Inc. has produced customized anti-idiotype (anti-ID) antibodies for the detection, identification, and analysis of therapeutic drugs to support pre-clinical and downstream clinical trials.  Anti-ID antibodies can be produced as polyclonal or hybridoma-based monoclonal antibodies depending on the needs of your study. These fit-for-purpose antibodies can be generated in a variety of hosts for use in studies to identify, neutralize, block, and assess the properties of drug to analyte binding on the target therapeutic antibody.

The idiotope is the unique set of antigenic determinants found within the variable region of an antibody. The idiotope region constitutes the paratope, or the antigen binding site, where the epitope portion of the analyte is bound. Analytes can be protein targets or drugs. When a second antibody is generated that specifically binds to the idiotope region of the therapeutic antibody, we refer to this antibody as an anti-idiotype or anti-idiotypic antibody. 
  Anti-ID_Diagram Figure 1. The functional parts of an antibody.







Types of Anti-ID Antibodies


Anti-idiotypic antibodies can be produced using proprietary methods developed at Rockland to tailor the specificity of the antibody to be fit-for-purpose for your needs. Type I anti-ID antibodies bind the therapeutic antibody at or near the idiotope preventing the therapeutic antibody from binding its target. Consequently, type I anti-ID antibodies are useful for identification purposes and for neutralizing studies. Type II anti-ID antibodies detect therapeutic antibodies regardless of whether or not it’s bound to its target and are non-inhibitory; therefore, not used for neutralization studies. Type III anti-ID antibodies only bind the therapeutic antibody when the therapeutic antibody is bound to its target and are most useful for pharmacodynamic and pharmacokinetic studies.

Type_1_Diagram1 Type_2_Diagram Type_3_Diagram
Type I
  • Inhibitory / neutralizing
  • Detects free antibody drug
 


Type II
  • Not inhibitory
  • Detects total antibody drug (free, partially bound, fully bound)
 
Type III
  • Antibody drug-target complex specific
  • Detects bound antibody drug only
                                

 

Figure 2. Types of anti-ID antibodies produced by Rockland. Anti-ID antibodies can be produced in a fit-for-purpose manner depending on how the antibody is ultimately to be deployed. Type I anti-ID antibodies are most useful for identification purposes and for neutralization studies. Type II anti-ID antibodies bind the therapeutic drug regardless of whether the analyte is bound. Type III anti-ID antibodies are most useful for PK and PD studies.




How are Anti-ID Antibodies Used?


Anti-ID antibodies are used various critical points of pre-clinical and clinical therapeutic drug manufacturing, testing, and release. Anti-ID antibodies are most often used in DRID testing for identification purposes and in ELISA assays for pharmacodynamic (PD) and pharmacokinetic assays (PK).


DRID1 DRID2
Figure 3. Double Radial Immunodiffusion (DRID) is a quantitative immunodiffusion technique used to identify, by observing the formation of precipitin arcs, the identity of various lots of therapeutic antibodies (outer wells) that react with a specific type I anti-idiotypic antibody (center well). ELISA is typically used in pharmacodynamic and pharmacokinetic assays using type II and type II anti-ID antibodies.


Anti-idiotypic antibodies are highly specialized anti-drug antibodies (ADA) that can be used for biological drug identification and for drug studies in PK assay to measure the levels of drug in patient samples. Anti-idiotypic antibodies recognize the idiotope of therapeutic antibodies. Most therapeutic antibodies are human in origin or have been “humanized;” therefore, epitopes unique for anti-ID antibodies recognize hypervariable regions of the therapeutic antibody. Rockland’s team of scientists can produce, purify, validate, and package anti-ID antibodies and develop assays and perform immunoassays to your precise requirements.




Related Services for Anti-Idiotypic Antibody Production


Rockland delivers services and provides solutions for diagnostic, pre-clinical and downstream clinical applications for government, private, biotechnology and pharmaceutical research laboratories.




Related Products for Anti-ID Antibodies 

 

Anti-idiotypic antibodies are commonly used in DRID testing, ELISA assays, and for PD and PK studies. Catalog reagents, especially buffers, blocking agents, cell lysates, enzyme substrates, and supporting reagents are most valuable when performing studies using anti-ID antibodies. 

 



 
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Anti-ID_Sellsheet
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