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Rockland Immunochemicals has collaborated with Bio-Rad to develop an enhanced multiplex fluorescent western blotting protocol. The assays produce high resolution immunoblots on PVDF membrane, providing increased visibility and profiling of low abundance proteins.
Rockland DyLight™ fluorochrome conjugated secondary antibodies are used to probe the membrane and the Molecular Imager® VersaDoc™ MP 4000 and the ChemiDoc™ XRS+ imaging system developed by Bio-Rad Laboratories, provides clear, accurate results. Several targets can be detected and imaged simultaneously, resulting in blots that show enhanced detection of distinct target bands labeled by a unique identifying color. Simultaneous detection helps cut time, while ensuring the target epitopes are still easily identified.
Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated
Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody
Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody
Rockland also collaborated with Bio-Rad on a two-dimensional workflow validation to develop an improved standardized immunoassay for HCP detection. This collaboration resulted in a method using a robust 2D SDS-PAGE using the PROTEAN® i12™ IEF System, ReadyStrip™ IPG Strips, and western blotting assay to validate the efficacy of immunocoverage for novel anti-HCP polyclonal antibodies. Validation is performed using BioRad’s PDQuest 2-D Analysis Software.
Rockland offers KCAJ07, an E.coli HCP Western Blot kit that contains two-dimensional validated antibodies, HRP conjugated secondary antibodies, blocking and wash solutions and E.coli control proteins. Rockland’s two-dimensional validated antibodies have been shown to react with more than 300 E.coli proteins from SDS/DTT solubilized E.coli cells.
Anti-Host Cell Protein (HCP) Antibody
E.coli Host Cell Protein Control Protein (HCP)
Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated
Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component system)
To learn more about HCP antibodies and these new systems or obtain a custom quote, contact Rockland today for information.
Download the Rockland and Bio-Rad Enhanced Multiplex Western Blot Poster
Download the Rockland and Bio-Rad HCP Development Blot Poster
Performance of a Western Blot:
ChemiDoc™ MP Introduction:
Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: firstname.lastname@example.orgPhone: 800.656.7625