Bio-Rad Collaboration

Rockland and Bio-Rad Collaborate on Enhanced Multiplex Western Blotting: Less Hassle. Better Resolution.


Rockland Immunochemicals has collaborated with Bio-Rad to develop an enhanced multiplex fluorescent western blotting protocol. The assays produce high resolution immunoblots on PVDF membrane, providing increased visibility and profiling of low abundance proteins.


Rockland DyLight™ fluorochrome conjugated secondary antibodies are used to probe the membrane and the Molecular Imager® VersaDoc™ MP 4000 and the ChemiDoc™ XRS+ imaging system developed by Bio-Rad Laboratories, provides clear, accurate results. Several targets can be detected and imaged simultaneously, resulting in blots that show enhanced detection of distinct target bands labeled by a unique identifying color. Simultaneous detection helps cut time, while ensuring the target epitopes are still easily identified.



Featured Products:


DyLight Conjugated Rabbit Antibody


Anti-RABBIT IgG (H&L) (GOAT) Antibody DyLight™ 488 Conjugated

GAPDH Rabbit Antibody


Anti-Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) (RABBIT) Antibody

Alpha Tubulin Mouse Antibody


Anti-alpha-Tubulin (MOUSE) Monoclonal Antibody

Blocking Buffers and Water


Blocking Buffers



Enhanced HCP ELISA Detection Using Antibodies Validated by Two-Dimensional (2D) Electrophoresis and Western Blotting



Rockland also collaborated with Bio-Rad on a two-dimensional workflow validation to develop an improved standardized immunoassay for HCP detection. This collaboration resulted in a method using a robust 2D SDS-PAGE using the PROTEAN® i12™ IEF System, ReadyStrip™ IPG Strips, and western blotting assay to validate the efficacy of immunocoverage for novel anti-HCP polyclonal antibodies. Validation is performed using BioRad’s PDQuest 2-D Analysis Software.


Rockland offers KCAJ07, an E.coli HCP Western Blot kit that contains two-dimensional validated antibodies, HRP conjugated secondary antibodies, blocking and wash solutions and E.coli control proteins. Rockland’s two-dimensional validated antibodies have been shown to react with more than 300 E.coli proteins from SDS/DTT solubilized E.coli cells.



Featured Products:


Host Cell Protein Antibody


Anti-Host Cell Protein (HCP) Antibody

CHP Control Proteins


E.coli Host Cell Protein Control Protein (HCP)

IgG Antibody Peroxidase Conjugated


Anti-RABBIT IgG (H&L) (GOAT) Antibody Peroxidase Conjugated

Chemiluminescent FemtoMax Substrate


Chemiluminescent FemtoMax™ Super Sensitive HRP Substrate for Microwell and/or Membrane (2 component system)



To learn more about HCP antibodies and these new systems or obtain a custom quote, contact Rockland today for information.

  Enhanced Multiplex Western Blot Poster 

Download the Rockland and Bio-Rad Enhanced Multiplex Western Blot Poster

HCP Development Poster 

Download the Rockland and Bio-Rad HCP Development Blot Poster

Bio-Rad Collaboration Videos:

Performance of a Western Blot:

View Performance of a Western Blot video

ChemiDoc™ MP Introduction:

View ChemiDoc™ MP video