Cell Culture Services


What is Cell Culture? 

 

Cell culture is the in vitro process by which cells, usually extracted from animals, are grown in a controlled environment. These cells are first removed from tissue and subsequently kept in favorable, controlled conditions. Cell culture material is produced from cell lines, both adherent and suspension. Typically flasks or cell factories are used for production.

 

Rockland's Cell Culture Services

 

At Rockland, our experience and capabilities enable us to provide customized services to meet our client's needs. We offer a number of different services from basic cell culture, large scale culture and production, and cell storage service, as well as monoclonal antibody production.  When combined these services form the basis for a complete package. Whatever stage in the research process, we are confident we can support your cell culture and cell line storage needs.

 

You can get a quote for any cell culture service here:

 

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  Cell Cultuer and Antibody Production

 

Cell Culture and Antibody Production

 

With over 30 years of experience in culturing various cell lines for antibody and protein production, we can produce cell culture material from both adherent and suspension lines.  Performing both adherent and suspension cell culture work, cells are routinely produced in T-Flask, roller bottles and stir tanks.  Hollow Fiber and CELLine flasks are designed to enhance small scale bio-production for antibody and protein generation. Other high capacity methods are also available for large scale production requirements.

 

ADHERENT CELL CULTURES - MAB-080

SUSPENSION CELL CULTURES - MAB-082

 

Wheaton CELLine

  • INTEGRA CELLINE CL-1000 FLASK - MAB-069
  • INTEGRA CELLINE CL-1000 FLASK (> FLASK) - MAB-070
 

 

STATIONARY FLASK CELL CULTURE

ROLLER BOTTLE CELL CULTURE

  • per 500 mL bottle per cell line (<1.0 L) - MAB-078
  • per Liter per cell line (1.0 - 2.0 L) - MAB-062
  • per Liter per cell line (>2.0 - 5.0 L) - MAB-063
  • per Liter per cell line (>5.0 - 20 L) - MAB-064
  • per Liter per cell line (>20 - 50 L) - MAB-075
  • per Liter per cell line (>50 L) - MAB-076
 
  Roller Bottle Cell Culture

 

BATCH BIOREACTOR - batch bioreactor production of antibody from CHO or hybridoma cells

  • 7 Liter BATCH BIOREACTOR -   MAB-031
  • 10 Liter BATCH BIOREACTOR  -  MAB-032
  • 15 Liter BATCH BIOREACTOR  -  MAB-033
  • 36 Liter BATCH BIOREACTOR  -  MAB-034    (inquire about other sizes)
  • MEDIA FOR BIOREACTOR BATCH PRODUCTION - Cost of medium, serum, or other additives used in batch bioreactor production of antibodies. - MAB-035

 

HOLLOW FIBER BIOREACTOR

  • 12 ml ECS Cartridge Start-Up Fee - MAB-065
  • 12 ml ECS Cartridge Weekly Fee - MAB-066
  • 60 ml ECS Cartridge Start-Up Fee - MAB-067
  • 60 ml ECS Cartridge Weekly Fee - MAB-068

 

SPINNER FLASK CELL CULTURE


 

Cell Banking Services/Cryopreservation

 

Custom Cell Banking services are flexible and allow us to meet client specific requests. Our custom services team researches and creates both master and working cell banks specific to each client's individual requirements.Cell line stability services can also be provided upon request.


RESEARCH CELL BANKING – Research cell banking is an inexpensive short term storage solution.  A set number of vials (10 or 25) are produced, tested for viability, stored and monitored in liquid nitrogen for 6 months

MASTER CELL BANKING - A set number of vials from 10 to 200 are produced, tested for viability, stored and monitored in liquid nitrogen for two (2) years


 

Cell Storage Service/Cryostorage

 

Cell storage for small and large cell banks can be provided on an annual basis.  Consider Rockland's robust cell storage facilities as a primary or secondary storage location for your most respected cell lines.  We urge you to protect your valuable research cell line by storage in multiple storage locations. 

 

LIQUID NITROGEN STORAGE - MAB-005

 

 


Cell Line Testing Service

 

Rockland can perform cell analysis, lysate production, mycoplasma and sterility for Rockland or client produced cell lines.

 

  • SUBCELLULAR FRACTIONATION - CUST48
  • CELL LYSATE SERVICE - MAB-081
  • MYCOPLASMA TEST for Cell Lines  - MAB-012
  • MAP TEST for cell line - MAB-013  

Cell Line Testing

 


 

Cell Viability Studies


We can perform cell viability and cell proliferation studies for Rockland or client produced cell lines. Each assay provides a unique snapshot of cell health. When combined, these assays can form the basis for cell viability and overall health.

 

  • Intracellular ThiolEZ™ Cell Survival Assay Kit - KLD-004
  • Oxphos™ Cell Survival Assay Kit -  KLD-002
  • RadChemDosi™ Cell Survival Assay Kit - KLD-003
  • CellCountEZ™ Cell Survival Assay Kit - KLD-001

 

 


 

Media Optimization Studies


Customized Media Optimization Studies are offered to include doubling times, viability and protein production parameters. Studies are fully customizable.

 

 

For information on Custom Monoclonal Antibody Production, click here.