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Humans have 58 known receptor tyrosine kinases (RTKs) which fall into 20 subfamilies. c-MET and RON are two receptor tyrosine kinases that belongs to the c-MET proto-oncogene family. c-Met and RON share similar structural and biochemical properties. Both proteins are heterodimers composed of a ~40Kda extracellular a-chain and a ~150 kDa transmembrane b-chain with intrinsic tyrosine kinase activity. c-Met is a receptor for hepatocyte growth factor (HGF), while RON is recognized by macrophage-stimulating protein (MSP), also known as HGF-like protein. c-Met and RON both are expressed by a variety of epithelial-derived tumors and cancer cell lines and thought to play a functional role in tumorigenesis and invasive-metastatic phenotypes. Both RON and c-Met are recent targets of drug studies to control cell migration and matrix invasion. Rockland Immunochemicals has developed a suite of research antibodies targeting activated and inactivated critical components of the c-MET signal pathway. These antibodies are designed to detect the phosphorylated and dephosphorylated forms of c-MET and RON, and their downstream adapter GAB1.
c-MET detection by Western blot using affinity purifiedpY1349pY1356 anti-c-Met antibody. The picture shows that phosphorylated c-Met is detected only in HGF-stimulated cells.
Detection of Gab1 usinganti-GAB1 antibody in the absence and presence of competing Gab1 peptide.
Detection of active RON pY1360 protein fragment usinganti-RONpY1360 antibody in the absence and presence of competing RONpY1360 peptide
For detection of total RON protein, pan-reactive rabbit polyclonal antibodies that have been raised against RON recombinant protein or rabbit polyclonal antibodies raised against peptide epitopes (RON against residues surrounding Y1360 and RON against residues surrounding Y1353 of human RON protein) is recommended. To detect various phosphorylated c-MET or RON or Gab1 isoforms, respective phospho-specific and site-specific antibodies are recommended. For example, RON phosphorylated at Y1353 position can be detected using phospho-specific RON pY1353 Antibody. For RON phosphorylated at Y1360 position, the phospho-specific RON pY1360 Antibody should be used. Similarly, to detect phosphorylation of c-MET at Y1349/Y1356 or Gab1 at Y659, c-Met phospho Y1349 / Y1356 Antibody or GAB1 pY659 Antibody should be used.
c-Met phospho Y1349/Y1356 Antibody (Rabbit)
RON Antibody (Rabbit)
RON phospho pY1360 Antibody (Rabbit)
RON phospho pY1353 Antibody (Rabbit)
GAB1 phospho pY659 Antibody (Rabbit)
c-MET Pathway Antibodies and ReagentsAbstractRockland have developed a suite of reagents to detect phosphorylated and inactive forms of c-MET and RON as well as their downstream adapter GAB1. These reagents are applicable for profiling c-MET pathway proteins and their phosphorylated stages in human cells for monitoring the efficacy of anti-cancer drugs. Applications Reagents for c-MET pathway pharmacodynamic study.Advantagesc-Met and RON both are expressed by a variety of epithelial-derived tumors and cancer cell lines and thought to play a functional role in tumorigenesis, invasive-metastatic phenotypes. Both RON and c-Met have become a drug target to control cell migration and matrix invasion. Developed antibodies enables to rapidly quantify c-MET family proteins and their phosphorylated stages in cells upon exposure to anticancer drugs, thereby allowing preclinical evaluation the anti-cancer drug efficacy and potential resistance problems.
To discuss licensing or custom services opportunities please contact:Dan KenneyDirector, Scientific Business Development(484) 791-3823
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Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625