Rockland's monoclonal antibody development services allow generation of high quality and target specific antibodies. As a complete service, Rockland can design, synthesize and conjugate peptides for use as immunogen for your monoclonal development program. Let Rockland’s expertise aid you in both the design and synthesis of antigenic peptides. Should you choose to utilize a recombinant protein as primary antigen, our expertise in mammalian, bacterial and insect cell-based recombinant protein expression will ensure production of antigen for immunization and screening regimen. If you choose to utilize a protein or live cell line as a primary antigen, Rockland’s molecular biology and cell culture services can support your needs.
Our list of services includes antigen consultation, peptide or protein antigen production, antibody generation, antibody purification and antibody conjugation to commonly used reporter molecules such as peroxidase or fluorochromes. When combined these services form the basis to create a custom antibody reagent for your specific immunoassay. Clients may select only antibody generation, or may choose to perform scale up, purification and more.
The phases for custom monoclonal antibody generation are shown below:
Each monoclonal project is performed in stages, and our clients can decide on when to proceed to each project stage. Rockland’s custom monoclonal antibody service can meet all of our customers’ specific needs. Whatever stage in the research process, we are confident we can support your custom antibody development requirements.You can get a quote for any antibody development service here:
Rockland can utilize many types of macromolecules as antigens providing the antigens do not constitute a biohazard. The antigen or immunogen may be a protein in the form of recombinant or purified protein, synthetic peptide, modified peptide, or whole cell lysates, or other types of macromolecules such as nucleic acids, lipids or carbohydrate complexes, and other variations. Custom peptide synthesis can be optimized through Peptide Design Consultation. We are experts in generating antibodies specific for post-translational modifications including phospho-epitopes, acetylation, methylation, hydroxylation, ubiquitinylation and more using modified peptides. Recombinant Protein Design and Preparation can be discussed with our molecular biology services team.
See Rockland’s Peptide Synthesis FAQ
Rockland has an optimized process to generate polyclonal antibody against phosphorylated epitopes and other post translational modifications of proteins. For instance, our phospho antibody generation process uses synthetic peptides. Rockland performs this service for both monoclonal and polyclonal projects.
Rockland has decades of experience in producing monoclonal antibodies in various host animals including mouse, rat and Armenian hamsters. We make use of electrofusion technology to produce large numbers of clones which allows us to be successful in producing monoclonal antibodies against the most difficult targets.
For all monoclonal antibody services and information requests please inquire with one of our experts
For additional information about Rockland’s monoclonal antibody development services click here :
Rockland offers several options for production of monoclonal antibodies. Our cell culture services offers tissue culture based protocols including flask and roller bottle. In addition we offer host-based ascites protocols that may use an outbred or an immunodeficient host system. Additionally purification services are available including low endotoxin purification methods.
Rockland can perform purification of antibody from generated antisera.
Cell Culture and Antibody Production Rockland has the capability to provide Cell Culture Services to meet our client's needs. We offer a number of different services from basic cell culture, large scale culture and production, and cell storage service as well as monoclonal antibody production.
RESEARCH CELL BANKING - Research cell banking is an inexpensive short term storage solution. A set number of vials (10 or 25) are produced, tested for viability, stored and monitored in liquid nitrogen for 6 months
MASTER CELL BANKING - A set number of vials from 10 to 200 are produced, tested for viability, stored and monitored in liquid nitrogen for two (2) years
Rockland offers multiple Antibody Conjugation formats for monoclonal and polyclonal antibodies
For a list of common questions such as antigen requirement see our Custom Services FAQs.