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Fluorescence is the property of certain substances to emit light. Emission occurs only after absorbing light or electromagnetic radiation at a specific wavelength. Emission light is usually of longer wavelength than the absorbed light. One of the most well-known natural fluorescent probes is Green Fluorescent Protein (GFP) from jellyfish. However, a fluorophore may also be a fluorescent organic compound usually with a low molecular weight (0.2-1 kDa) and typically containing numerous aromatic groups, or plane or cyclic moieties with π bonds. Fluorophores, i.e. either fluorescent proteins or organic dyes, can be directly conjugated to antibodies making them ideal for use in various immunoassays.
Goat IgG (H&L) Antibody ATTO 425 Conjugated Pre-Adsorbed Rabbit Polyclonal
Rabbit IgG (H&L) Antibody DyLight™ 488
Conjugated Pre-Adsorbed Goat Polyclonal
GST Antibody Fluorescein Conjugated Rabbit Polyclonal
Fluorescent labeled antibodies can be used to detect target proteins in fluorescent western blots, or dot blots, in FLISA assays, as well as immunofluorescent microscopy, flow cytometry and immunohistochemistry. By using the right combination of fluorescent labeled antibodies multiple targets can be visualized simultaneously in a single assay (fluorescent multiplexing).
Primary, or direct, immunofluorescence uses a single antibody conjugated directly to a fluorescent dye. Secondary, or indirect, immunofluorescence uses two antibodies; a primary antibody which recognizes the target biomolecule and binds to it and a secondary antibody conjugated to a fluorescent dye, which recognizes and binds to the primary antibody and indirectly localizes the target for detection. Fluorescent dyes are optimized for excitation and detection in the visible light to near infrared spectrum, 400 nm - 800 nm. The choice of fluorochrome to be used is influenced both by the application and the excitation wavelengths available. The table below provides an overview of fluorescent dies used at Rockland.
Rockland conjugates a broad group of secondary antibodies to many of the classic fluorescent markers including fluorescein, rhodamine, Texas Red, CyDyes™ and Phycoerythrin (RPE). All of the conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and more. Rockland also produces many next generation fluorochrome dyes designed for detection of primary antibodies in multiplex, multi-color analysis. Next generation fluorochrome conjugates (Atto-tec dyes, DyLight™ dyes) offer superior absorption (high extinction coefficient), high fluorescence quantum yield, and superior high photostability.
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Rockland Immunochemicals Inc.Limerick, PA 19468E-mail: email@example.comPhone: 800.656.7625