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Blocking Buffer for
Fluorescent Western Blotting
Click here to view the Western Blot Processor BlotCycler Video
Rockland Immunochemicals and Precision Biosystems have used fluorescent labeled secondary antibodies to compare manual and automated processing. The multi-color visualization presented on the poster demonstrates that BlotCycler™ is able to produce clear and high quality results, allowing a straightforward process for the identification of individual protein.Using two identical protocols, we have showed that BlotCycler™ increases the signal to noise ratio by reducing background and increasing signal intensity. BlotCycler™ provides extremely good reproducibility that is probably related to minimal variability in washing and incubation times. BlotCycler™ can be used to optimize the concentrations of primary and secondary antibodies potentially providing huge saving by decreasing the amount of antibody used.Thus BlotCycler™ offers a time-saving alternative to manual processing of fluorescent western blot by providing very low background staining, high signal-to-noise ratio, ability to multiplex detection and excellent reproducibility. BlotCycler™ provides opportunity for easy optimization of western blotting and significantly improves the quality of the procedure.
HeLa Whole Cell Lysate
Anti-Mouse IgG (H&L) DyLight™ 549
Anti-Rabbit IgG (H&L) DyLight™ 649
IL-2 Human Recombinant Protein
NORMAL GOAT SERUM (NGS)
Blocking Buffer for Fluorescent Western Blotting
Blotto Immunoanalytical Grade (Non-Fat Dry Milk)
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625