Antibodies for Signal Enhancement


Antibodies for Signal Enhancement Overview

Rockland provides a series of reagents which provide unique mechanism for signal enhancement and antibody bridging.  Signal enhancing antibodies increase the overall size of the antigen-antibody complex and results in increased signal strength and assay sensitivity.  An antibody bridging event occurs when an antibody is recognized by a second labeled antibody thereby presenting an additional detection mechanism. Signal enhancing antibodies typically target reporter molecules such as peroxidase, KLH or luciferase proteins.   Signal enhancing antibodies are frequently using with immunohistochemistry products.





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Enhancing or Boosting Peroxidase detection

Horseradish Peroxidase (HRP) is an enzyme that is utilized in molecular biology to convert various substrates to detectable compounds.  Anti-peroxidase is a valuable tool for common immunoassays including immunoprecipitation (IP), ELISA, western blotting (WB) and immunohistochemistry (IHC). The effective application of immunohistochemical methods on formalin-fixed samples often uses the immunoperoxidase bridge method or the peroxidase anti-peroxidase (PAP) complex method which form large insoluble complexes and is useful for amplifying the signal in immunohistochemistry.


 Peroxidase-anti-Peroxidase Complex


Peroxidase-anti-Peroxidase Complex



Peroxidase Antibodies



Product Name   Catalog #   Applications
 Anti-PEROXIDASE (Horseradish) (GOAT) Antibody   100-1138   IHC, WB, ELISA
 Anti-PEROXIDASE (Horseradish) (MOUSE) Antibody   100-3138   IHC, WB, ELISA
 Anti-PEROXIDASE (Horseradish) (RABBIT) Antibody   100-4138   IHC, WB, ELISA
 Anti-PEROXIDASE (Horseradish) (RABBIT) Antibody Biotin Conjugated   200-4638   IHC, WB, ELISA


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Enhancing or Boosting Luciferase Detection

Fluorescent proteins are versatile markers for monitoring physiological processes, visualizing protein localization, and detecting transgenic expression. However, in some cases either the natural signal alone is insufficient and requires enhancement or the recombinant protein has been denatured and is no longer fluorescent.  Firefly luciferase bioluminescence color varies between yellow-green to red. Luciferase is commonly transfected into cells and used as a reporter in transcriptional activity assay.  Luciferase is also used to measure amounts of ATP in cell viability assays or used in kinase activity assays.  Lastly Luciferase may be used in whole animal imaging.


Luciferase Antibodies


Product Name   Catalog #   Applications
 Anti-LUCIFERASE (GOAT) Antibody   100-101-150   WB, IP, ELISA
 Anti-LUCIFERASE (GOAT) Antibody Fluorescein Conjugated   200-102-150   WB, IP, ELISA
 Anti-LUCIFERASE (GOAT) Antibody Peroxidase Conjugated   200-103-150   WB, IP, ELISA
 Anti-LUCIFERASE (GOAT) Antibody Biotin Conjugated   200-106-150   WB, IP, ELISA


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Enhancing or Boosting KLH Detection

Keyhole limpet hemocyanin (KLH) is a large metal-containing protein consisting of 400 kDa subunits that is frequently used carrier protein in immunization procedures.  KLH is most typical conjugated to a small molecule, peptide, or protein thereby rendering the complex highly immunogenic (see Rockland’s custom conjugation service). KLH has been used as a carrier for vaccines and antigens and as adjuvant treatment in regimens such as antimicrobial therapy.  Rockland’s anti-KLH antibody allows for the detection of KLH, important for monitoring immune Response, infection and inflammation.


KLH Antibodies



Product Name   Catalog #   Applications
Anti-KLH (MOUSE) Monoclonal Antibody   200-301-E66   WB, IP, ELISA
Anti-KLH (RABBIT) Antibody Rhodamine Conjugated   600-400-466   WB, IP, ELISA
Anti-KLH (RABBIT) Antibody   600-401-466   WB, IP, ELISA
Anti-KLH (RABBIT) Antibody Fluorescein Conjugated   600-402-466   WB, IP, ELISA
Anti-KLH (RABBIT) Antibody Peroxidase Conjugated   600-403-466   WB, IP, ELISA
Anti-KLH (RABBIT) Antibody Biotin Conjugated   600-406-466   WB, IP, ELISA


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The Rockland Antibody for Signal Enhancement Advantage    

Signal enhancement antibodies can be used but are not limited to the following assays:
• Immunoprecipitation
• Western blot
• Immunofluorescence

See Rockland’s epitope tag antibodies for anti-FLAG and anti-GST antibodies.

Rockland offers custom molecular biology services for the production or recombinant proteins containing Luciferase, GFP, RFP, or other fusion protein constructs.   See all of Rockland’s custom services.




   TrueBlot IP/Western Blot Mouse Rabbit Goat


Loading Control Kit

Loading Control Kit  

Images Generated with Rockland's Loading Kit


The Loading Control Antibody Sampler Kit contains antibodies to a variety of housekeeping proteins. Loading controls are essential for the interpretation of western blot data. Loading control antibodies allow the verification of equal protein loading between samples. Ideal loading controls are expressed constitutively and at high levels with low variability between cell lines and experimental conditions. Also, the loading control should be at a different molecular weight than the protein of interest this allows the bands to be visually distinguishable. The Loading Control Antibody Sampler kit contains enough primary and secondary antibodies to perform ten western blots per loading control antibody.