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Stem cell research remains a growing area of scientific exploration, with numerous possibilities in various medical fields. Rockland maintains a large collection of reagents for bioimaging, immunofluorescence microscopy, multiplexed protein assays, ELISA, and Western blot analysis. These antibodies and antibody based tools include a rapidly expanding portfolio of flow cytometry reagents as well as antibodies, buffers, and protocols for cell signaling analysis. Research in developmental biology has led to the discovery of several types of stem cells, including embryonic stem cells, embryonic germ cells and adult stem cells.
Oct-4 is a protein that is critically involved in the self-renewal of undifferentiated embryonic stem cells and is used as a marker for undifferentiated cells. Oct-4 expression must be closely regulated as too much or too little will cause differentiation of the cells. Rockland's Oct 4 antibody recognizes the pluripotency marker Oct4, expressed in Embryonic Stem Cells and Embryonic Germ Cells. Protect your experiments with Rockland antibodies. Compromise elsewhere.
Anti-Nestin (RABBIT) Antibody
Secondary antibody conjugates are ideal for various immunofluorescence based assays including fluorescent western blotting, immunofluorescence microscopy, FLISA, and Flow Cytometry. Rockland conjugates a broad group of secondary antibodies to many of the classic and next generation fluorescent markers including fluorescein, Texas Red, and Phycoerythrin, providing depth and breadth for today’s high level research. Learn more about secondary antibody conjugates at the links below.
The Mouse TrueBlot® Western Blot Kit contains the critical supporting reagents, buffers, and substrates for Stem Cell immunoprecipitation and Western blotting of samples using TrueBlot second step immunoblotting reagents in conjunction with your own primary IP antibody and primary (Mouse IgG) Western blotting antibody. TrueBlot technology enables unhindered detection of protein bands of interest which would otherwise be obscured by the presence of reduced and denatured heavy and light chain immunoglobulin in the blot (as detected by the conventional immunoblotting HRP anti-mouse IgG reagent). Mouse IgG TrueBlot® ULTRA is the unique horseradish peroxidase conjugated anti-mouse IgG immunoblotting second step reagent which enables detection of Stem Cell immunoblotted target protein bands, without hindrance by interfering immunoglobulin heavy and light chains from your IP antibody. Use it in place of your usual HRP anti-mouse IgG immunoblotting second step reagent. It is easy to generate publication-quality Stem Cell IP Western Blot data with Mouse IgG TrueBlot® ULTRA.
Mouse IgG TrueBlot ULTRA is ideal for use in protocols involving immunoblotting of immunoprecipitated proteins. TrueBlot preferentially detects the non-reduced form of mouse IgG over the reduced, SDS-denatured form of IgG. When the immunoprecipitate is fully reduced immediately prior to SDS-gel electrophoresis, reactivity of Mouse IgG TrueBlot ULTRA with the 55 kDa heavy chains and the 23 kDa light chains of the immunoprecipitating antibody is minimized thereby eliminating interference by the heavy and light chains of the immunoprecipitating antibody in Stem Cell IP Western Blotting applications. Applications include studies examining post-translational modification (e.g., phosphorylation or acetylation) or protein-protein interactions.
Rockland Immunochemicals Inc.Gilbertsville, PA 19525E-mail: email@example.comPhone: 800.656.7625