RFP Antibody Peroxidase Conjugated Pre-Adsorbed

Datasheet

RFP Antibody Peroxidase Conjugated Pre-Adsorbed

Name: RFP Antibody Peroxidase Conjugated Pre-Adsorbed
Brand: Rockland Immunochemicals
Price: 449.0 USD
Availability: InStock

Rabbit Polyclonal

1 References

Item No. 600-403-379

Size: 100 µg

Applications: WB, ELISA, IF

Reactivity: RFP, rRFP

Host Species: Rabbit

$449.00 /Per Item

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Background

Fluorescent proteins such as Discosoma Red Fluorescent Protein (DsRed) from sea anemone Discosoma sp. mushroom or green fluorescent protein (GFP) from Aequorea victoria jellyfish are widely used in research practice. Fusion RFP and GFP commonly serve as marker for gene expression and protein localization. As DsRed and GFP share only 19% identity, therefore, in general, anti-GFP antibodies do not recognize DsRed protein and vice versa. Structurally, Discosoma red fluorescent protein is similar to Aequorea green fluorescent protein in terms of its overall fold (a β-can) and chromophore-formation chemistry. However, Discosoma red fluorescent protein undergoes an additional step in the chromophore maturation and obligates tetrameric structure. Rockland offers many controls, monoclonal, and polyclonal antibodies for RFP.

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Specifications for RFP Antibody Peroxidase Conjugated Pre-Adsorbed

Product Details

Description: Anti-RFP (RABBIT) Antibody Peroxidase Conjugated (Min X Hu Ms & Rt Serum Proteins) - 600-403-379

Synonyms: rabbit anti-RFP antibody peroxidase conjugation, rabbit anti-Red Fluorescent Protein antibody peroxidase conjugation, HRP conjugated rabbit anti-RFP antibody, DsRed, rDsRed, Discosoma sp. Red Fluorescent Protein, Red fluorescent protein drFP583

Host Species: Rabbit

Conjugate: Peroxidase (HRP)

Clonality: Polyclonal

Format: IgG

Target Details

Gene Name: DsRed - View All DsRed Products

Reactivity: RFP, rRFP

Immunogen Type: Recombinant Protein

Immunogen: The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma.

Purity/Specificity: Rabbit Anti-RFP Peroxidase Conjugated Antibody was prepared from monospecific rabbit antiserum by immunoaffinity chromatography using Red Fluorescent Protein (Discosoma) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities. Expect reactivity against RFP and its variants: mCherry, tdTomato, mBanana, mOrange, mPlum, mOrange and mStrawberry. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-peroxidase, anti-Rabbit Serum and purified and partially purified Red Fluorescent Protein (Discosoma). No reaction was observed against Human, Mouse or Rat serum proteins. ELISA was used to confirm specificity at less than 0.1% of target signal.

Database Links:

UniProtKB - Q9U6Y8

Application Details

Tested Applications: ELISA, WB

Suggested Applications: IF - View References

Application Note: Rabbit Anti-RFP Peroxidase Conjugated Antibody has been tested in ELISA and Western blot. It can be used to detect RFP by ELISA (sandwich or capture) for the direct binding of antigen or to detect RFP or RFP containing proteins on Western blots. References suggest that the antibody is also suitable for IHC. Optimal titers for applications should be determined by the researcher.

Formulation

Physical State: Lyophilized

Concentration: 1.0 mg/mL by UV absorbance at 280 nm

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer: 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Reconstitution Volume: 100 µL

Reconstitution Buffer: Restore with deionized water (or equivalent)

Shipping & Handling

Shipping Condition: Ambient

Storage Condition: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiration: Expiration date is one (1) year from date of receipt.

References (1)

Bohlen MO, El-Nahal HG, Sommer MA. (2019). Transduction of Craniofacial Motoneurons Following Intramuscular Injections of Canine Adenovirus Type-2 (CAV-2) in Rhesus Macaques. Front Neuroanat.

Applications

IF, Confocal Microscopy

PubMed

Related Protocols

Adherent Cell Lysis Protocol

ELISA Protocol

Fluorescent Western Blotting Protocol

Histone Immunoblotting Protocol

Immunocytochemistry (ICC) Protocol

Immunofluorescence (IF) Protocol

In-Cell Western (ICW) Protocol

IP-WB with TrueBlot® Protocol

Multi-Lysate Western Blotting Protocol

Nuclear & Cytoplasmic Extract Protocol

Sandwich ELISA Protocol for Collagen

Suspension Cultured Cell Lysis Protocol

Western Blotting (WB) Protocol

Certificate of Analysis Lookup

Disclaimer

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.