Kits  >  Antibodies to GFP and Antibodies to RFP

GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting

Monoclonal 9F9.F9
NCI Collaboration JCYIA Product
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  • Rockland Anti GFP antibody Western Blot Kit: for GFP Chemiluminescent  Western Blotting-GFP Western Blot
  • Rockland Anti GFP antibody Western Blot Kit: for GFP Chemiluminescent  Western Blotting
  • Rockland Anti GFP antibody Western Blot Kit: for GFP Chemiluminescent  Western Blotting
  • Rockland Anti GFP antibody Western Blot Kit: for GFP Chemiluminescent  Western Blotting
1 Kit
Ships within 2 weeks.

GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting Properties

GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting - KCA215
Known Cross Reactivity
wt GFP from Aequorea victoria, rGFP, eGFP
Monoclonal 9F9.F9
Western Blot : Yes
Other Dilution: User Optimized
Product Type
Kit Type
Chemiluminescent Western Blot Kit
Physical State
Shipping Condition
Wet Ice
Wash buffers MUST NOT contain SODIUM AZIDE or other inhibitors of peroxidase activity!
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GFP Western Blot Kit: for GFP Chemiluminescent Western Blotting Description

GFP Chemiluminescent Western Blot Kit allows for the detection of GFP-tagged recombinant proteins present in cell lysates provided by the user. After protein separation by SDS-PAGE and transfer, the membrane is probed with monoclonal Anti-GFP. Detection of the membrane bound antibody-antigen complex is achieved by the addition of a secondary antibody conjugated to the enzyme horseradish peroxidase. The enzyme reacts with a specialized formulation of luminol, an extremely sensitive, non-radioactive substrate that emits light and allows visualization using X-ray film or other imaging methods, including highly sensitive CCD cameras and imaging systems.
GFP Western Blotting Kit, GFP Chemiluminescent Kit, Green Fluorescent Protein Antibody Kit, Immunoblotting Kit, GFP, rGFP, eGFP
Storage Condition
See GFP Western Blot kit insert for complete instructions.
Application Note
GFP Western Blot Kit is ideal for detection of GFP-tagged recombinant proteins by western blot. The GFP Western Blot kit makes use of Rockland's optimized anti-GFP antibody. The Chemiluminescent GFP Western Blot kit is useful for both western blotting and dot blotting methods. Please read the entire product insert prior to use.
Rockland Immunochemicals’ Chemiluminescent Western Blot Kit for GFP combines all of the necessary reagents with a rapid proven protocol and the extremely high signal detection of our luminol chemiluminescent substrate for the detection of recombinant proteins containing GFP and its variants. The Chemiluminescent Western Blot Kit design includes straightforward procedures and color-coding to allow for ease of use. This kit contains sufficient substrate for up to 30 mini blots at 7.5 x 8 cm2 (1,800 cm2) and is stable for at least 1 year when stored as indicated.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Antibodies, A Laboratory Manuel. Ed Harlow and David Lane, eds. Cold Spring Harbor Press. 1988. Chapter 12 gives an excellent overview of Western Blotting techniques, including India Ink staining. Current protocols in Molecular Biology. J. Ausebel, et al, eds. John Wiley and Sons, New York. Gives a complete protocol of Western Blotting and Dot Blotting. Molecular Cloning: A Laboratory Manuel. 2nd Edition. J. Sambrook, E.F. Fritsch and T. Maniatis, eds. Cold Spring Harbor Press, 1989. Chapter 18 gives detailed protocols for both the production of cell lysates and electrophoresis and blotting of proteins. Antibodies, A Practical Approach. 2nd Edition. Catty, D., ed. IRL Press, Oxford, England. 1990. Volumes I and II represent a detailed and complete reference for most current antibody techniques.
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Product Type
GFP and Epitopes;
Catalog Number

Product Type
GFP and Epitopes;
Reacts With
wt, rGFP, eGFP
Catalog Number

Product Type
Secondary Antibodies;
Reacts With
Catalog Number

Product Label


Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry