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Avidin, Biotin, Streptavidin > Avidin-Biotin Reagents

STREPTAVIDIN PEROXIDASE Conjugated

Number:

S000-03

Size:

1 mg

Price:

$161.00

Shipping:

$32.00

Handling:

$20.00

Availability:

Ships next business day.

Datasheet
References
Reviews
Protocols
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STREPTAVIDIN PEROXIDASE Conjugated Properties

Description

STREPTAVIDIN PEROXIDASE Conjugated - S000-03

Application

ELISA : 1:20,000 - 1:200,000
Western Blot : 1:10,000 - 1:40,000
Immunohistochemistry: 1:1,000 - 1:5,000
Other Dilution: User Optimized

Reagent Type

Streptavidin

Physical State

Lyophilized

Shipping Condition

Ambient

Label

Peroxidase (Horseradish)

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Reconstitution Volume

1.0 mL

Reconstitution Buffer

Restore with deionized water (or equivalent)

Preservative

0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!

Stabilizer

10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free

Relevant Links

UniProtKB - P22629
NCBI - CAA00084.1

Download Datasheet

Download CofA:

Lot#

STREPTAVIDIN PEROXIDASE Conjugated Description

Background

Streptavidin is isolated from bacteria, Streptomyces avidinii, and has an exceptionally high binding affinity for B7 (biotin). Rockland offers streptavidin in unconjugated and conjugated forms for common immunoassays including ELISA, western blotting, immunohistochemistry. Streptavidin is a tetrameric protein capable of binding 4 biotin groups to each molecule of streptavidin. While streptavidin has identical binding properties as avidin, it lacks the glycoprotein portion of the molecule and therefore shows less non-specific binding. Streptavidin is a slightly smaller molecule with a molecular weight of approximately 53.6 kDa. The sequence of avidin only shows 30% homology with streptavidin, and anti-avidin and anti-streptavidin antibodies are not immunologically cross reactive. Horseradish Peroxidase (HRP) is an enzyme that utilize organic peroxide compounds as electron donors. Naturally provides protection for plants against pathogens, but can be utilized in molecular biology to convert various substrates to detectable compounds (such as in Western Blotting and ELISAs).

Synonyms

HRP-SA, Horseradish Peroxidase conjugated S avidin, streptococcus avidin, Streptavidin HRP, Streptavidin conjugated to horseradish peroxidase (HRP), HRP-linked Streptavidin

Storage Condition

Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. Streptavidin Peroxidase conjugated is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Streptavidin Peroxidase is a useful detection reagent for primary antibodies conjugated to biotin. Streptavidin Peroxidase can be utilized in Immunohistochemistry, Western Blotting, and ELISA experiment formats in combination with the proper substrate (TMB-1000 or FEMTOMAX-110).

Purity/Specifity

Streptavidin Peroxidase was prepared from chromatographically purified streptavidin. Streptavidin Peroxidase was assayed by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase and anti-Streptavidin.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference

Specific Reference

Phillips, A. T., Rico, A. B., Stauft, C. B., Hammond, S. L., Aboellail, T. A., Tjalkens, R. B., & Olson, K. E. (2016). ENTRY SITES OF VENEZUELAN AND WESTERN EQUINE ENCEPHALITIS VIRUSES IN THE MOUSE CENTRAL NERVOUS SYSTEM FOLLOWING PERIPHERAL INFECTION. Journal of virology, JVI-03219.