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Primary Antibodies > Stem Cell Antibodies

IL-8 Antibody

Rabbit Polyclonal

Western Blot of Rabbit anti-Human IL-8 Antibody.
Lane 1: Recombinant Human IL-8.
Load: 50 ng per lane.
Primary antibody: Rabbit anti-Human IL-8 Antibody 1:1,000 overnight at 4°C.
Secondary antibody: HRP Goat-a-rabbit secondary antibody (p/n 611-103-122) at 1:40,000 for 30 min at RT.
Block: MB-070 for 30 min at RT.
Predicted/Observed size: 11 kDa, 11 kDa for Human IL-8.

Number:

109-401-311

Size:

1 mL

Price:

$319.00

Shipping:

$20.00

Handling:

$20.00

Availability:

Ships next business day.

Datasheet
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IL-8 Antibody Properties

Description

Anti-Human IL-8 (RABBIT) Antibody - 109-401-311

Host

Rabbit

Target Species

Human

Known Cross Reactivity

Human

Clonality

Polyclonal

Application

ELISA : 1:1,000 - 1:5,000
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:400 - 1:800
ImmunoPrecipitation: 1:400 - 1:800
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping Condition

Dry Ice

Format

Antiserum

Label

Unconjugated

Concentration

75 mg/mL by Refractometry

Buffer

None

Preservative

None

Stabilizer

None

GeneName

IL8

IL-8 Antibody Description

Background

Interleukin-8 is a chemokine produced by macrophages and other cell types such as epithelial cells. Human IL-8 (CXCL8) antibody is commonly used in many experiments focused around inflammation. Anti-IL-8 antibody is ideal for investigators involved in Cancer, Immunology and Cardiovascular research.

Synonyms

Anti-CXCL 8, CXCL8 antibody, 310C antibody, AMCF 1 antibody, AMCF1 antibody, b ENAP antibody, Beta thromboglobulin like protein antibody, CXC chemokine ligand 8 antibody, nterleukin8 antibody, K60 antibody, LECT antibody, LUCT antibody

Immunogen

The whole rabbit serum was prepared by repeated immunizations with recombinant human IL-8 produced in E.coli.

Immunogen Type

Recombinant Protein

Storage Condition

Store anti-IL8 antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

IL 8 antibody has been tested for use in ELISA and recognizes IL-8 found in cells, supernatants and other body fluids. Reactivity in other immunoassays is unknown.

Purity/Specifity

Anti-IL 8 antiserum has been heated to 56°C for 30 minutes. The antiserum detects recombinant and native IL-8 present in body fluids and cell supernatants in ELISA. Although likely to be functional, this antiserum has not been evaluated in other assays, including neutralization and immunohistochemistry.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference