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Primary Antibodies > Sample Size Primary Antibodies

THRA Antibody

Rabbit Polyclonal

Code

600-401-A38S

Size

25 µL

Price

$95.00

Availability

Ships next business day.

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Western blot using Rockland's affinity purified anti-THRA antibody shows detection of purified recombinant THRA (lane 1) and THRA present in a 293 cell lysate after transient transfection with THRA (lane 3). No staining is evident in lysates from mock-transfected 293 cells (lane 2). Endogenous THRA is not detected in mouse brain whole cell lysate (lane 4). Nuclear extracts may be required to detect endogenous THRA as the protein localizes within the nucleus. The band at ~55 kDa, indicated by the arrowhead, corresponds to THRA. Personal communication, S. Cheng and H. Ying, NCI, Bethesda, MD.

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Synonyms: Thyroid hormone receptor alpha Nuclear receptor subfamily 1 group A member 1 c-erbA-alpha c-erbA-1 V-erbA-related protein 7 EAR-7 TRa1

Datasheet
References
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THRA Antibody Properties

Description

Anti-Thyroid Hormone Receptor alpha (THRA) (RABBIT) Antibody - 600-401-A38S

Host

Rabbit

Target Species

Human

Known Cross Reactivity

Human

Clonality

Polyclonal

Application

ELISA : 1:300,000
IF Microscopy : 1:200
Western Blot : 1:500 - 1:2,000
Immunohistochemistry: 1:200
ImmunoPrecipitation: 1:100
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

0.89 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

THRA

THRA Antibody Description

Background

This antibody is designed, produced, and validated as part of a collaboration between Rockland and the National Cancer Institute (NCI) and is suitable for Cancer, Immunology and Nuclear Signaling research. Thyroid hormone receptor alpha is a nuclear hormone receptor with high affinity for the hormone triiodo-thyronine. THRA is one of the several receptors for thyroid hormone, and has been shown to mediate the biological activities of thyroid hormone. Knockout studies in mice suggest that the different receptors, while having a certain extent of redundancy, may mediate different functions of thyroid hormone. THRA interacts with NCOA3 and NCOA6 co-activators, leading to a strong increase in transcription of target genes. THRA is localized within the nucleus and has been found to exist as 4 isoforms originating from alternative splicing variants. Isoform 1 has a distinct C-terminus compared to isoform 2.

Immunogen

This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a region near the amino terminus of human THRA isoform 1 protein.

Immunogen Type

Peptide

Storage Condition

Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Application Note

This affinity purified antibody has been tested for use in ELISA and western blotting.

Purity/Specifity

This product was affinity purified from monospecific antiserum by immunoaffinity chromatography. This antibody reacts with human THRA protein and recognizes THRA isoform 1. A BLAST analysis was used to suggest cross-reactivity with THRA from mouse, human and rat based on a 100% homology with the immunizing sequence. Cross-reactivity with THRA from other sources and isoforms may occur but has not been determined.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.

General Reference

Ying,H., Araki,O., Furuya,F., Kato,Y. and Cheng,S.Y. (2007) Impaired adipogenesis caused by a mutated thyroid hormone alpha1 receptor. Mol. Cell. Biol. 27 (6), 2359-2371. Nascimento,A.S., Dias,S.M., Nunes,F.M., Aparicio,R., Ambrosio,A.L., Bleicher,L., Figueira,A.C., Santos,M.A., de Oliveira Neto,M., Fischer,H., Togashi,M., Craievich,A.F., Garratt,R.C., Baxter,J.D., Webb,P. and Polikarpov,I. (2006) Structural rearrangements in the thyroid hormone receptor hinge domain and their putative role in the receptor function. J. Mol. Biol. 360 (3), 586-598. Wan,W., Farboud,B. and Privalsky,M.L. (2005) Pituitary resistance to thyroid hormone syndrome is associated with T3 receptor mutants that selectively impair beta2 isoform function. Mol. Endocrinol. 19 (6), 1529-1542.

Specific Reference

López-Juárez, A., Remaud, S., Hassani, Z., Jolivet, P., Pierre Simons, J., Sontag, T., ... & Demeneix, B. A. (2012). Thyroid Hormone Signaling Acts as a Neurogenic Switch by Repressing Sox2 in the Adult Neural Stem Cell Niche. Cell stem cell, 10(5), 531-543.

Antigen Retrieval Methods

Azide Removal by Dialysis

Beta Galactosidase Conjugates

Biotin Binding Assay (HABA)

Biotin Conjugates

Cell Lysates Protocol

CyDye™ Conjugates

DyLight™ Antibody Conjugate Properties

DyLight™ Antibody Spectra

ELISA

ELISA to Quantify Collagen

Extended Conjugate Information Sheet