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Primary Antibodies > Loading Control Antibodies

beta Actin Antibody

Rabbit Polyclonal

Number:

600-401-886

Size:

200 µg

Price:

$301.00

Shipping:

$41.00

Handling:

$20.00

Availability:

Ships next business day.

Datasheet
References
Reviews
Protocols
Related Products

beta Actin Antibody Properties

Description

Anti-beta Actin (RABBIT) Antibody - 600-401-886

Host

Rabbit

Target Species

Human

Known Cross Reactivity

Mouse, Rat, Human, Monkey, Sheep, Bovine, Goat, Guinea Pig, Swine, Hamster

Clonality

Polyclonal

Application

ELISA : 1:10,000 - 1:40,000
IF Microscopy : 1:500 - 1:2,000
Western Blot : 1:1,000 - 1:4,000
Other Dilution: User Optimized

Physical State

Liquid (sterile filtered)

Shipping Condition

Dry Ice

Format

IgG

Label

Unconjugated

Concentration

1.0 mg/mL by UV absorbance at 280 nm

Buffer

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.01% (w/v) Sodium Azide

Stabilizer

None

GeneName

ACTB

Relevant Links

NCBI - 4501885
UniProtKB - P60709

Download Datasheet

Download CofA:

Lot#

beta Actin Antibody Description

Background

In vertebrates 3 main groups of actin isoforms, alpha, beta and gamma have been identified. Alpha actins are found in muscle tissues and are a major constituent of the contractile apparatus. Beta and gamma actins coexist in most cell types as components of the cyto-skeleton and as mediators of internal cell motility. Beta actins are highly conserved proteins that are involved in cell motility, structure and integrity. Beta actins are cytoplasmic proteins. Anti-Actin is a loading control antibody and is critical for the correct interpretation of your western blot. Beta-Actin Loading Control Antibody is used to normalize the levels of protein detected by confirming that protein loading is uniform across the gel.

Synonyms

rabbit anti-beta Actin Antibody, Actin Antibody, Loading Control Antibody, beta actin, ß actin, anti-beta actin antibody

Immunogen

beta-Actin Loading Control Antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 359-368 of Human beta Actin.

Immunogen Type

Peptide

Storage Condition

Store beta-Actin Loading Control Antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Application Note

Anti-beta Actin Antibody has been tested for use in ELISA, immunohistochemistry and western blot. Specific conditions for reactivity should be optimized by the end user. Beta actin present in fibroblast connective tissue stains very brightly. Beta actin present in neuromuscular junctions also stains. Paraformaldehyde fixation yields brighter staining than formalin or methanol fixation. Expect a band at ~42 kDa in size corresponding to beta actin by western blotting in the appropriate cell lysate or extract.

Purity/Specifity

Anti-beta-Actin Loading Control Antibody reacts with Human, Rat, Monkey and Mouse. beta-Actin Loading Control Antibody is expected to cross-react with a wide range of species due to sequence homology. Anti-Beta Actin is affinity-purified antibody is directed against human beta Actin protein. A BLAST analysis was used to suggest that this antibody would react with beta Actin from a wide range of organisms, including most vertebrates and some yeast. Broad reactivity makes this antibody an excellent loading control.

Disclaimer Note-General

This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

General Reference

Hu,P., Wu,S. and Hernandez,N. (2004) A role for beta-actin in RNA polymerase III transcription. Genes Dev. 18 (24), 3010-3015. Dahlen,A., et al. (2004) Activation of the GLI oncogene through fusion with the beta-actin gene (ACTB) in a group of distinctive pericytic neoplasms: pericytoma with t(7;12). Am. J. Pathol. 164 (5), 1645-1653. Navarro-Lerida,I., Martinez Moreno,M., Roncal,F., Gavilanes,F., Albar,J.P. and Rodriguez-Crespo,I. (2004) Proteomic identification of brain proteins that interact with dynein light chain LC8. Proteomics 4 (2), 339-346.

Specific Reference

Hopkins MM, Feng X, Liu M, Parker LP, Koh DW. (2015) Inhibition of the transient receptor potential melastatin-2 channel causes increased DNA damage and decreased proliferation in breast adenocarcinoma cells. Int J Oncol. 2015 May;46(5):2267-76. doi: 10.3892/ijo.2015.2919. Epub 2015 Mar 6. Khoh-Reiter S, Jessen BA. (2009) Evaluation of the cytotoxic effects of ophthalmic solutions containing benzalkonium chloride on corneal epithelium using an organotypic 3-D model. BMC Ophthalmol. 2009 Jul 28;9:5. doi: 10.1186/1471-2415-9-5.