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Primary Antibodies  >  Transcription Factor Antibodies
 

NFKB p65 Antibody

Rabbit Polyclonal
Code

100-4165
Size

100 µL
Price

$309.00
Availability

Ships next business day.
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Immunohistochemistry of NFKB p65 (Rel A) antibody  
Tissue: lymphocytes and germinal center cells of the tonsil
Fixation:  formalin fixed paraffin embedded  
Antigen retrieval:  user optimized
Primary antibody:  NFKB p65 (Rel A) antibody 1:400 
Secondary antibody:  Peroxidase goat anti-rabbit at 1:10,000 for 45 min at RT
Localization:  nuclear and occasionally cytoplasmic
Staining:  Moderate positive nuclear or cytoplasmic staining was observed in lymphocytes and germinal center cells of the tonsil.
Western Blot of Rabbit anti-NFkB p65 (Rel A) antibody.  
Lane 1:  HeLa cell extract.  
Lane 2:  HeLa cell extract.  
Load:  35 µg per lane.
Primary antibody:  NFkB p65 Rel A antibody at 1:5000 for 2 H at RT.
Secondary antibody:  Peroxidase rabbit secondary antibody at 1:2000 for 60 min at RT.
Block:  5% BLOTTO 2 H at RT.
Predicted/Observed size:  ~65 kDa, ~65 kDa for NFkb p65 Rel A. 
Other band(s): None.
Shipping & Handling Fees Apply.

Shipping:
$85.00 to United States
Handling:
$20.00
Synonyms: NFKB, nfkb, NF-kB, NF-kappaB, NFkappaB, Nuclear factor NF-kappa-B p65 subunit

NFKB p65 Antibody Properties

Description
Anti-NFKB p65 (Rel A) (RABBIT) Antibody - 100-4165
Host
Rabbit
Target Species
Human
Known Cross Reactivity
human, mouse, rat
Clonality
Polyclonal
Application
ELISA : 1:5,000
GelShift : 05 µL - 1.0 µL
Western Blot : 1:2,000 - 1:5,000
Immunochemistry: 1:400
Other Dilution: User Optimized
Physical State
Liquid (sterile filtered)
Shipping condition
Dry Ice
Format
Antiserum
Label
Concentration
80 mg/mL by Refractometry
Buffer
None
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
None
GeneName
RELA
Download CofA
Lot#  

NFKB p65 Antibody Description

Background
Anti-NFkB p65 Antibody recognizes NFKB p65 which is a component of NFKB. NFKB was originally identified as a factor that binds to the immunoglobulin kappa light chain enhancer in B cells. It was subsequently found in non-B cells in an inactive cytoplasmic form consisting of NFkappaB bound to IkappaB. NFkappaB was originally identified as a heterodimeric DNA binding protein complex consisting of p65 (RelA) and p50 (NFKB1) subunits. Other identified subunits include p52 (NFKB2), c-Rel, and RelB. The p65, cRel, and RelB subunits are responsible for transactivation. The p50 and p52 subunits possess DNA binding activity but limited ability to transactivate. p52 has been reported to form transcriptionally active heterodimers with the NFkappaB subunit p65, similar to p50/p65 heterodimers. The heterodimers of p52/p65 and p50/p65 are regulated by physical inactivation in the cytoplasm by IkappaBalpha. IkappaBalpha-??binds to the p65 subunit, preventing nuclear localization and DNA binding. Low levels of p52 and p50 homodimers can also exist in cells.
Immunogen
NFkB p65 (Rel A) peptide corresponding to a region near the C-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).
Immunogen Type
Peptide
Storage Condition
Store NF-kappaB antibody at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
Application Note
Anti-NFkB p65 (Rel A) is suitable for the detection by immunoblot of human and mouse NFkB p65 (Rel A), immunohistochemistry, and ELISA.  This product was also tested in a gel supershift assay and found to be reactive against all p65 (Rel A) containing human, mouse or rat NFkB complexes using 0.5 to 1.0 µl per assay.
Purity/Specifity
NFkB p65 (Rel A) was prepared from monospecific antiserum by delipidation and defibrination.  Anti-NFkB p65 (Rel A) may react non-specifically with other proteins.  Control peptide (code #100-4165p) will compete only with the specific reaction of antiserum with the NFkB p65 (Rel A) subunit.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 326, Gilbertsville, Pennsylvania, USA.
General Reference
Specific Reference
Unlap, M.T. and Jope, R.S. (1997). Dexamethasone attenuates NF-?B DNA binding activity without inducing I?B levels in rat brain in vivo. Molecular Brain Research 45: 83-89. Poser, I., Golob, M., Buettner, R. and Bosserhoff, A.K. (2003) Upregulation of HMG1 Leads to Melanoma Inhibitory Activity Expression in Malignant Melanoma Cells and Contributes to Their Malignancy Phenotype. Mol. Cell Biol. 23(8): 2991-2998. Ghosh M, Yang Y, Rothstein JD, Robinson MB. Nuclear factor-?B contributes to neuron-dependent induction of glutamate transporter-1 expression in astrocytes. J. Neurosci. 2011 Jun 22;31(25):9159-9169. Jobin, C, et al. (1999) Curcumin blocks cytokine-mediated NF-kappa B activation and proinflammatory gene expression by inhibiting inhibitory factor I-kappa B kinase activity. J. Immunol. 163(6): 3474-3483. Cao S, Zhang X, Edwards JP, Mosser DM. (2006 ) NF-kappaB1 (p50) homodimers differentially regulate pro- and anti-inflammatory cytokines in macrophages. J. Biol. Chem. 2006 Sep 8;281(36):26041-26050. Lou H, Kaplowitz N. (2007 ) Glutathione depletion down-regulates tumor necrosis factor alpha-induced NF-kappaB activity via IkappaB kinase-dependent and -independent mechanisms. J. Biol. Chem. 2007 Oct 5;282(40):29470-29481. Yoon C, Korade Z, Carter BD.(2008) Protein kinase A-induced phosphorylation of the p65 subunit of nuclear factor-kappaB promotes Schwann cell differentiation into a myelinating phenotype. J. Neurosci. 2008 Apr 2;28(14):3738-3746. Begley LA, Kasina S, Mehra R, et al. (2008) CXCL5 promotes prostate cancer progression. Neoplasia. 2008 Mar;10(3):244-254. Limpert Allison S. ; Carter BD. (2010) Axonal Neuregulin 1 Type III Activates NF-kappa B in Schwann Cells during Myelin Formation. Journal Biological Chemistry 285 (22): 16614-16622 DOI: 10.1074/jbc.M109.098780 Published: MAY 28 2010
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, chicken
Catalog Number
100-401-401

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-4164

Product Type
Primary Antibodies;
Applications
ELISA, WB, GS, Other
Reacts With
human
Catalog Number
100-4166

Product Type
Primary Antibodies;
Applications
ELISA, WB
Reacts With
human
Catalog Number
100-4184

Product Label

 

Description
Unconjugated
Color
Conjugation Reference
Molecular Weight
Excitation Wavelength
Conjugation Chemistry