Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed
Western Blot of Anti-Rabbit IgG (H&L) (GOAT) Antibody (Min X Bv, Ch, Gt, GP, Ham, Hs, Hu, Ms, Rt & Sh Serum Proteins) (p/n 611-101-122)
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Datasheet

Rabbit IgG (H&L) Secondary Antibody Fluorescein Conjugated Pre-Adsorbed

Goat Polyclonal

8 References
611-102-122
1 mg
WB, IF, Dot Blot, FISH, Multiplex
Rabbit
Goat
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Background

Anti-Rabbit secondary antibody conjugated to FITC is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.

Product Details

Goat Anti-Rabbit IgG (H&L) Antibody Fluorescein Conjugated (Min X Bv Ch Gt GP Ham Hs Hu Ms Rt & Sh Serum Proteins) - 611-102-122
Goat anti-Rabbit IgG Antibody fluorescein Conjugation, Goat anti-Rabbit IgG FITC Conjugated Antibody
Goat
IgG (H&L)
Fluorescein (FITC)
Polyclonal
IgG

Target Details

Rabbit
Rabbit IgG whole molecule
Secondary antibody conjugate was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.   Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Goat Serum, Rabbit IgG and Rabbit Serum.  No reaction was observed against Bovine, Chicken, Goat, Guinea Pig, Hamster, Horse, Human, Mouse, Rat or Sheep Serum Proteins.

Application Details

Dot Blot, WB
FISH, IF, Multiplex  - View References
Anti-Rabbit IgG Fluorescein Conjugate has been tested by dot blot and western blot. When choosing a secondary antibody product, consideration must be given to species and immunoglobulin specificity, conjugate type, fragment and chain specificity, level of cross-reactivity, and host-species source and fragment.

Formulation

Lyophilized
1.0 mg/mL by UV absorbance at 280 nm
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
0.01% (w/v) Sodium Azide
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
1.0 mL
Restore with deionized water (or equivalent)

Shipping & Handling

Ambient
Store secondary antibody at 4° C prior to restoration.   For extended storage aliquot antibody and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use.
Expiration date is one (1) year from date of receipt.
(). The ubiquitin ligase Ozz decreases the replacement rate of embryonic myosin in myofibrils. Physiol Rep.
Applications
IF, Confocal Microscopy
(). TGF-β1 Provides Neuroprotection via Inhibition of Microglial Activation in 3-Acetylpyridine-Induced Cerebellar Ataxia Model Rats. Front Neurosci.
Applications
WB, IB, PCA
(). HPV integration hijacks and multimerizes a cellular enhancer to generate a viral-cellular super-enhancer that drives high viral oncogene expression. PLOS Genetics
Applications
ISH, FISH
(). Resveratrol attenuates denervation-induced muscle atrophy due to the blockade of atrogin-1 and p62 accumulation. International Journal of Medical Sciences
Applications
IF, Confocal Microscopy
(). Toxoplasma gondii infection induces the formation of host's nuclear granules containing poly(A)-binding proteins. Can J Microbiol.
Applications
IF, Confocal Microscopy
(). Human Papillomavirus Integration: Analysis by Molecular Combing and Fiber‐FISH. Curr Protoc Microbiol.
Applications
ISH, FISH
(). In-vivo quantification of the revascularization of a human acellular dermis seeded with EPCs and MSCs in co-culture with fibroblasts and pericytes in the dorsal chamber model in pre-irradiated tissue. Cell Tissue Bank.
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IF, Confocal Microscopy
(). Polycation‐based DNA complexes for tumor‐targeted gene delivery in vivo. J Gene Med.
Applications
IF, Confocal Microscopy; Multiplex Assay

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This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.

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