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GOAT IgG (H&L) Antibody IRDye800® Conjugated Pre-adsorbed

Donkey Polyclonal
NCI Collaboration
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Western Blot showing detection of alpha tubulin from HeLa and NIH/3T3. Protein was run on a 4-20% gel, transferred to 0.45 µm nitrocellulose.  After blocking with 1% BSA-TTBS (p/n MB-013, diluted to 1X) 30 min at 20°C, primary antibody was used at 1:2500 overnight at 4°C.  IRDye800CW®  secondary antibody was used at 1:20,000 in Blocking Buffer for Fluorescent Western Blotting (p/n MB-070) and imaged on the LiCor Odyssey imaging system.  Arrow indicates correct 50 kDa molecular weight position expected for alpha tubulin.
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Number:
605-732-125
Size:
500 µg
Price:
$199.00
Shipping:
$40.00
Handling:
$20.00
Availability:
Ships next business day.

GOAT IgG (H&L) Antibody IRDye800® Conjugated Pre-adsorbed Properties

Description
Anti-GOAT IgG (H&L) (DONKEY) Antibody IRDye® 800 Conjugated (Min X Ch GP Ham Hs Ms Rb & Rt Serum Proteins) - 605-732-125
Host
Donkey
Specificity
IgG (H&L)
Target Species
Goat
Clonality
Polyclonal
Application
FLISA : 1:5,000 - 1:10,000
Western Blot : 1:10,000 - 1:25,000
Other Dilution: User Optimized
Physical State
Lyophilized
Shipping Condition
Ambient
Format
IgG
Label
FP Value
4.6 moles IRDye® 800 per mole of IgG
Concentration
1.0 mg/mL by UV absorbance at 280 nm
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Reconstitution Volume
500 µL
Reconstitution Buffer
Restore with deionized water (or equivalent)
Preservative
0.01% (w/v) Sodium Azide
Stabilizer
10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
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GOAT IgG (H&L) Antibody IRDye800® Conjugated Pre-adsorbed Description

Background
This product is designed for immunofluorescence microscopy, fluorescence based plate assays (FLISA) and fluorescent western blotting. This product is also suitable for multiplex analysis, including multicolor imaging, utilizing various commercial platforms.
Immunogen
Goat IgG whole molecule
Storage Condition
Store vial at 4° C prior to restoration.   For extended storage aliquot contents and freeze at -20° C or below.  Avoid cycles of freezing and thawing.  Centrifuge product if not completely clear after standing at room temperature.  This product is stable for several weeks at 4° C as an undiluted liquid.  Dilute only prior to immediate use. 
Application Note
Fluorescence technology is widely used to detect proteins. However, many common visible fluorophores often result in considerable background fluorescence in the visible range. Visible fluorophores are rarely used for membrane-based protein detection because of this high background. IRDye® 800 and IRDye® 700DX antibody and reagent conjugates are specifically designed for protein detection methods that use longer-wavelength, near-infrared (IR) fluorophores to visualize proteins in western blotting and other applications. Very low background fluorescence in the IR range provides for a much higher signal-to-noise ratio than visible fluorophores. Detection levels in the picogram range on Western blots rival the sensitivity of chemiluminescence on film. IRDye® 800 conjugates are optimized for the Odyssey® Infrared Imaging System developed by LI-COR. IRDye® 800 conjugates are also suitable for immunofluorescence microscopy using commercially available excitation/emission filters in the 780nm/820nm range. Dual simultaneous labeling in western blots or microscopy is achieved when IRDye® 800 conjugates are used in conjunction with IRDye® 700DX or DyLight™680 conjugates. IRDye® 800 and IRDye® 700DX conjugates provide an ultra-sensitive and convenient alternative to standard chemiluminescent protein detection methods, as well as a valuable tool for multicolor imaging.
Purity/Specifity
This product was prepared from monospecific antiserum by immunoaffinity chromatography using Goat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.  Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Donkey Serum, Goat IgG and Goat Serum.  No reaction was observed against Chicken, Guinea Pig, Hamster, Horse, Mouse, Rabbit or Rat Serum Proteins.
Disclaimer Note-General
This product is for research use only and is not intended for therapeutic or diagnostic applications. Please contact a technical service representative for more information. All products of animal origin manufactured by Rockland Immunochemicals are derived from starting materials of North American origin. Collection was performed in United States Department of Agriculture (USDA) inspected facilities and all materials have been inspected and certified to be free of disease and suitable for exportation. All properties listed are typical characteristics and are not specifications. All suggestions and data are offered in good faith but without guarantee as conditions and methods of use of our products are beyond our control. All claims must be made within 30 days following the date of delivery. The prospective user must determine the suitability of our materials before adopting them on a commercial scale. Suggested uses of our products are not recommendations to use our products in violation of any patent or as a license under any patent of Rockland Immunochemicals, Inc. If you require a commercial license to use this material and do not have one, then return this material, unopened to: Rockland Inc., P.O. BOX 5199, Limerick, Pennsylvania, USA.
General Reference
Specific Reference
Sandhir, R; Berman, NEJ. Age-dependent response of CCAAT/enhancer binding proteins following traumatic brain injury in mice. Neurochemistry International 56 (2010) 188–193
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Product Type
Primary Antibodies;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
human, mouse, rat, monkey
Catalog Number
200-301-268

Product Type
GFP and Epitopes;
Applications
ELISA, WB, IHC, IF, FC
Reacts With
wt and all variants such as rGFP, eGFP, S65T-GFP, RS-GFP, YFP and EGFP
Catalog Number
600-401-215

Product Type
Supporting Reagents;
Applications
ELISA, FLISA, WB, IHC, IF, IP, FC
Catalog Number
B304

Product Type
Supporting Reagents;
Applications
WB
Catalog Number
MB-070

Product Label

 

Description
IRDye® 800
Color
Infra-Red
Conjugation Reference
Modified from LI-COR Biosciences, Lincoln, NE
Molecular Weight
1166
Excitation Wavelength
778
Conjugation Chemistry
N-hydroxysuccinimide (NHS) ester